... bridges called ring canals. As the egg chamber grows, the germline cells and the ring canals that connect them increase in size. Here, we demonstrate that ring canal size scaling is related to lineage; the largest, ‘first-born’ ring canals increase in size at a relatively slower rate than ring canals...

...Ronit S. Kaufman; Kari L. Price; Katelynn M. Mannix; Kathleen M. Ayers; Andrew M. Hudson; Lynn Cooley ABSTRACT Animal germ cells communicate directly with each other during gametogenesis through intercellular bridges, often called ring canals (RCs), that form as a consequence of incomplete...

...Katelynn M. Mannix; Rebecca M. Starble; Ronit S. Kaufman; Lynn Cooley ABSTRACT Gametogenesis is dependent on intercellular communication facilitated by stable intercellular bridges connecting developing germ cells. During Drosophila oogenesis, intercellular bridges (referred to as ring canals; RCs...

...Andrew M. Hudson; Katelynn M. Mannix; Julianne A. Gerdes; Molly C. Kottemann; Lynn Cooley ABSTRACT During Drosophila oogenesis, specialized actin-based structures called ring canals form and expand to accommodate growth of the oocyte. Previous work demonstrated that Kelch and Cullin 3 function...

...Åsmund Husabø Eikenes; Lene Malerød; Anette Lie-Jensen; Catherine Sem Wegner; Andreas Brech; Knut Liestøl; Harald Stenmark; Kaisa Haglund In many organisms, germ cells develop as cysts in which cells are interconnected via ring canals (RCs) as a result of incomplete cytokinesis. However...

... identified key Src64-dependent mechanisms that regulate actin cytoskeletal dynamics during the growth of actin-rich ring canals, which act as intercellular bridges between germ cells. By contrast, the molecular mechanisms that regulate Src64 activity levels and potential roles for Src64 in additional...

... that microtubules are required for three forms of particle movement within the nurse cells, while transport through the ring canals linking the nurse cells and oocyte appears to be independent of both microtubules and actin filaments. As particles enter the oocyte, a final microtubule-dependent step directs...

...Margaret de Cuevas; Allan C. Spradling ABSTRACT The Drosophila oocyte develops within a cyst of 16 germline cells interconnected by ring canals. Polarized, microtubule-based transport of unknown determinants is required for oocyte formation, but whether polarity is established during or after cyst...

... to the developing oocyte. Analysis of Src64 egg chambers showed defects in the ring canals that interconnect the oocyte and its 15 associated nurse cells. Src64 ring canals fail to accumulate the high levels of tyrosine phosphorylation that are normally present. Despite the reduced tyrosine phosphorylation, known...

... is regulated in a tissue-specific manner causing the ratio of full-length to ORF1 protein to vary in different tissues. The only detected defect for kelch mutants is female sterility, and kelch protein is localized to the ovarian ring canals. kelch mutant ring canals are disorganized and have partly occluded...