... micromeres. Most mesoderm cells later return by transfating, but pigment cells do not. Single-cell RNA sequencing, tracked over time, reveals the reprogramming sequence of those replacements. Beginning with an early endoderm specification state, cells progress through endomesoderm, then mesoderm, and finally...

... evidence supporting the concept that meiosis opposes aging via various mechanisms for cells to regain totipotency and start a new life cycle. Holliday Marchantia Cellular aging Meiosis Reprogramming Austrian Science Fund http://dx.doi.org/10.13039/501100002428 P36231...

...Masaki Yagi; Joy E. Horng; Konrad Hochedlinger ABSTRACT Cellular plasticity progressively declines with development and differentiation, yet these processes can be experimentally reversed by reprogramming somatic cells to induced pluripotent stem cells (iPSCs) using defined transcription factors...

...J. Raúl Perez-Estrada; Jared A. Tangeman; Maeve Proto-Newton; Harshavardhan Sanaka; Byran Smucker; Katia Del Rio-Tsonis ABSTRACT During tissue regeneration, proliferation, dedifferentiation and reprogramming are necessary to restore lost structures. However, it is not fully understood how...

...: (1) reprogramming, in which mature, specialised cells undergo a reversal of their developmental state, regaining pluripotency to then adopt a new cellular identity and function; (2) programming from pluripotent cells or progenitors, where less-committed cells are guided towards specific cell fates...

... of Biologists Ltd 2023 Summary: This Review provides an overview of the remarkable roles that metabolism and metabolic switches play during development, differentiation and regeneration. Metabolic switch Embryonic development Postnatal development Regeneration Reprogramming Stem cells...

... stem cells and their overexpression in more committed progenitors drives reprogramming to a naïve, self-renewing stem cell-like state. Crucially, individual Hox family members are expressed in a location-specific manner and their stem cell-promoting activity is only observed when the Hox gene...

... Reprogramming Transcription factor Gene duplication Pseudogene Evolution Biotechnology and Biological Sciences Research Council http://dx.doi.org/10.13039/501100000268 BBS/E/B/000C0421 BBS/E/B/000C0422 Medical Research Council https://dx.doi.org/10.13039/501100000265 MR/T011769/1...

... reprogramming contributes significantly to the dynamic gene expression during ZGA; however, the mechanism has yet to be resolved. Here, we find histone deacetylases 1 and 2 (HDAC1/2) can regulate ZGA through lysine deacetylase activity. Notably, in mouse embryos, overexpression of a HDAC1/2 dominant-negative...

... and MYC (OSKM) enables reprogramming of differentiated cells into pluripotent embryonic stem cells. Methods based on partial and reversible in vivo reprogramming are a promising strategy for tissue regeneration and rejuvenation. However, little is known about the barriers that impair reprogramming...

... Gandhi , S. , Ezin , M. and Bronner , M. E. ( 2020 ). Reprogramming axial level identity to rescue neural-crest-related congenital heart defects . Dev. Cell 53 , 300 - 315.e4 . 10.1016/j.devcel.2020.04.005 Grande , M. T. , Sánchez-Laorden , B. , López-Blau , C...

.... In terms of developmental potential, a totipotent cell differs from a pluripotent cell by its ability to develop into an entire organism by itself (see Box 1 and the accompanying piece by Riveiro and Brickman, 2020 ). Totipotency is established concomitantly with epigenetic reprogramming after...

... morphology and fate-marker expression in embryos in which suspensor division was activated by different triggers to address the developmental paths towards reprogramming. Our results show that reprogramming of Arabidopsis suspensor cells towards embryonic identity is a specific cellular response...

... important in ensuring tissue integrity. Over the past decade, several approaches to experimentally reprogram cell fate have emerged. Importantly, efforts to improve and understand these approaches have uncovered novel molecular determinants that reinforce lineage commitment and help resist cell fate changes...

... expression and conversion of ∼40% of mature MG into a neuronal/RPC phenotype. Our results suggest that the differences in miRNA expression between MG and RPCs contribute to their difference in neurogenic potential, and that manipulations in miRNAs provide a new tool with which to reprogram MG for retinal...

...Michelle K. Y. Seah; Yaju Wang; Pierre-Alexis Goy; Hui Mun Loh; Wen Jun Peh; Diana H. P. Low; Brenda Y. Han; Esther Wong; Ei Leen Leong; Gernot Wolf; Slim Mzoughi; Heike Wollmann; Todd S. Macfarlan; Ernesto Guccione; Daniel M. Messerschmidt ABSTRACT Global epigenetic reprogramming is vital to purge...

... using microfluidics and mRNA for reprogramming ( Giulitti et al., 2019 ). He highlighted how this method allowed them to generate human iPSCs that have a naïve-like state (i.e. naïve iPSCs; niPSCs). They are currently trying to understand why the microfluidic system is so much more efficient...

... Reprogramming Tissue repair Wound healing Schweizerischer Nationalfonds zur Förderung der Wissenschaftlichen Forschung 10.13039/501100001711 31003A_169930 Ministerio de Economía, Industria y Competitividad 10.13039/501100010198 BFU2015-67623-P Leibniz-Gemeinschaft...

... of reprogrammed and differentiated cells. Plat-E cells used for generating retrovirus were obtained from Cell Biolabs (catalogue number RV-101). The presence of blasticidin- and puromycin-resistance genes, which were inserted into the cell line to allow antibiotic selection for the presence...

... disease-specific alterations in methylation patterns. In contrast, foetal-derived organoids underwent drastic methylation changes in vitro , suggesting an in vitro maturation that could be used to model epithelial development. One of the most striking examples of plasticity is the reprogramming...