... but not sufficient for repression of B-myb transcription in quiescent fibroblasts . Oncogene 13 , 1073 - 1082 . Bettencourt-Dias , M. , Giet , R. , Sinka , R. , Mazumdar , A. , Lock , W. G. , Balloux , F. , Zafiropoulos , P. J. , Yamaguchi , S. , Winter , S. , Carthew...

... of the ureter. Using misexpression and loss-of-function mice combined with molecular profiling approaches, we show that Tbx18 is required and sufficient to repress metanephric mesenchymal gene programs. We identify Wt1 as a functional target of TBX18. Our work suggests that TBX18 acts as a permissive factor...

...Megan E. Dowdle; Sookhee Park; Susanne Blaser Imboden; Catherine A. Fox; Douglas W. Houston; Michael D. Sheets ABSTRACT Bicaudal-C (Bicc1) is a conserved RNA-binding protein that represses the translation of selected mRNAs to control development. In Xenopus embryos, Bicc1 binds and represses...

... more than one, with Drosophila Brinker (Brk) recruiting the co-repressors CtBP and Groucho (Gro), in addition to possessing a third repression domain, 3R. Previous studies indicated that Gro is sufficient for Brk to repress targets in the wing, questioning why it should need to recruit CtBP, a short...

...Qi Dai; Celia Andreu-Agullo; Ryan Insolera; Li Chin Wong; Song-Hai Shi; Eric C. Lai The activity of the Notch pathway revolves around a CSL-class transcription factor, which recruits distinct complexes that activate or repress target gene expression. The co-activator complex is deeply conserved...

...Yun Li; Jean Z. Maines; Ömür Y. Tastan; Dennis M. McKearin; Michael Buszczak In the Drosophila ovary, bone morphogenetic protein (BMP) ligands maintain germline stem cells (GSCs) in an undifferentiated state. The activation of the BMP pathway within GSCs results in the transcriptional repression...

... ), whereas the boundaries of the stripes are formed by Hunchback (Hb)- and Knirps (Kni)-mediated repression ( Clyde et al., 2003 ; Small et al., 1996 ; Yu and Small, 2008 ). However, other mechanisms of activation must exist because mutations that remove components of the JAK-STAT pathway do not completely...

... F. , Basler K. ( 2007 ). An optimized transgenesis system for Drosophila using germ-line-specific {varphi}C31 integrases . Proc. Natl. Acad. Sci. USA 104 , 3312 . Cai H. N. , Arnosti D. N. , Levine M. ( 1996 ). Long-range repression in the Drosophila embryo...

... degradation. This implies that phosphorylation of Gro interferes in some other manner with its ability to repress. Fig. 5. Maternal expression of Groucho or its derivatives does not disrupt embryonic anteroposterior axis formation. The anterior localisation of hb ( A-D ) and posterior...

... these interactions might affect target gene activation or repression. We find that two Smad proteins, effectors of the Drosophila Dpp/TGF-β pathway, that are genetically required for the activation of the spalt ( sal ) gene in the wing,collaborate with the Hox protein Ultrabithorax (Ubx) to directly repress sal...

...). PcG genes are essential genes in higher eukaryotes responsible for the maintenance of the spatially distinct repression of developmentally important regulators such as the homeotic genes. Their absence, as well as overexpression, causes transformations in the axial organization of the body. Although...

...Stephanie E. Winter; Gerard Campbell Patterning along developing body axes is regulated by gradients of transcription factors, which activate or repress different genes above distinct thresholds. Understanding differential threshold responses requires knowledge of how these factors regulate...

... was also detected in utricles(data not shown). The observed increase in gene expression, prior to utricle formation,suggests that transcription of such genes is normally inhibited by HR. To determine whether expression of the upregulated genes is normally repressed by HR, we examined expression...

...Paolo Struffi; Maria Corado; Meghana Kulkarni; David N. Arnosti The Drosophila Knirps protein is a short-range transcriptional repressor that locally inhibits activators by recruiting the CtBP co-repressor. Knirps also possesses CtBP-independent repression activity. The functional importance...

... in all nuclei anterior to the stripe 2 position. The gap gene giant ( gt ) is involved in a repression mechanism that sets the anterior stripe border, but genetic removal of gt (or deletion of Gt-binding sites) causes stripe expansion only in the anterior subregion that lies adjacent to the stripe border...

... Xmyf-5 ( Xtmyf-5 ) genomic DNA fragment that accurately recapitulates the expression of the endogenous gene. Deletion and mutational analysis has identified HBX2, an essential element, approximately 1.2 kb upstream from the start of transcription, which is necessary for both activation and repression...

... transcriptional repressor with a minimal 36 amino acid repression domain which can mediate promoter-specific repression by suppressing the activity of homeodomain-containing activator proteins. Mutations in HESX1 associated with pituitary disease appear to modulate the DNA-binding affinity of HESX1 rather than...

... ranging from sex determination to embryonic patterning and neuroblast specification. We have characterized a function of Gro in imaginal development, namely the repression of hedgehog ( hh ) in anterior wing pouch cells. hh encodes a secreted morphogen with potent patterning activities. In Drosophila...

... by VP16-Gsc was fully rescued by Frzb, a secreted Wnt inhibitor, indicating that activation of ectopic Wnt signaling was responsible, at least in part, for the anterior defects. Supporting this idea, Xwnt8 expression was activated by VP16-Gsc in animal explants and the dorsal marginal zone, and repressed...

...Rubén Darío Flores-Saaib; Songtao Jia; Albert J. Courey ABSTRACT In the Drosophila embryo, Dorsal, a maternally expressed Rel family transcription factor, regulates dorsoventral pattern formation by activating and repressing zygotically active fate-determining genes. Dorsal is distributed...