...Meng Yu; Konrad Thorner; Sreeja Parameswaran; Wei Wei; Chuyue Yu; Xinhua Lin; Raphael Kopan; Matthew R. Hass ABSTRACT The conserved Runt-related (RUNX) transcription factor family are master regulators of developmental and regenerative processes. Runx1 and Runx2 are expressed in satellite cells...

... of blast colony development, as a haemangioblast-like branch point from which SOX17 − CD34 + CD43 + blood cells and SOX17 + CD34 + CD43 − endothelium subsequently arose. Most human blood cell development was dependent on RUNX1. Deletion of RUNX1 only permitted a single wave of yolk sac-like primitive...

...Jana Koth; Xiaonan Wang; Abigail C. Killen; William T. Stockdale; Helen G. Potts; Andrew Jefferson; Florian Bonkhofer; Paul R. Riley; Roger K. Patient; Berthold Göttgens; Mathilda T. M. Mommersteeg ABSTRACT Runx1 is a transcription factor that plays a key role in determining the proliferative...

... that is strictly dependent on the transcription factor RUNX1. Although it is well established that RUNX1 is essential for the onset of hematopoiesis, little is known about the role of RUNX1 dosage specifically in hemogenic endothelium and during the endothelial-to-hematopoietic transition. Here, we used the mouse...

...). The transcription factor Runx1 is required for EHT, but for how long and which endothelial cells are competent to respond to Runx1 are not known. Here, we show that the ability of Runx1 to induce EHT in non-hemogenic endothelial cells depends on the anatomical location of the cell and the developmental age...

... transcription. Although the factors regulating EHT in hemogenic endothelium (HE) of the dorsal aorta have been relatively well studied, the molecular regulation of yolk sac HE remains poorly understood. Here, we show that SOX7 inhibits the expression of RUNX1 target genes in HE, while having no effect on RUNX1...

... cells that will undergo EHT, and the cellular events enabling this transition, are not known. We set out to define precisely the morphological events of EHT, and to correlate cellular morphology with the expression of the transcription factors RUNX1 and SOX17. A novel strategy was developed to allow...

... through sequential upregulation of CD41 and CD45 followed by emergence of fully functional definitive HSCs. Although largely dispensable at later stages, the Runx1 transcription factor is crucially important during developmental maturation of HSCs; however, exact points of crucial involvement of Runx1...

...Joanna Tober; Amanda D. Yzaguirre; Eileen Piwarzyk; Nancy A. Speck The transcription factor Runx1 is essential for the formation of yolk sac-derived erythroid/myeloid progenitors (EMPs) and hematopoietic stem cells (HSCs) from hemogenic endothelium during embryogenesis. However, long-term...

... expression leads to cancers and developmental disorders. We conducted a forward genetic screen in zebrafish for positive regulators of runx1 that yielded the cohesin subunit rad21 . Zebrafish embryos lacking Rad21, or cohesin subunit Smc3, fail to express runx3 and lose hematopoietic runx1 expression...

...Maggie L. Kalev-Zylinska; Julia A. Horsfield; Maria Vega C. Flores; John H. Postlethwait; Maria R. Vitas; Andrea M. Baas; Philip S. Crosier; Kathryn E. Crosier RUNX1/AML1/CBFA2 is essential for definitive hematopoiesis, and chromosomal translocations affecting RUNX1 are frequently involved in human...