... implications for the trajectory of an RNA as it exits the nucleus. Moreover, we quantify the stoichiometric relationships between germ granule compartments and RNA to reveal discrete populations of nuage that assemble in a hierarchical manner and differentially associate with RNAi-targeted transcripts...

... suppression of siRNA production helps to distinguish self versus nonself siRNAs in C. elegans germline and adds complexity to RNAi-mediated epigenetics. siRNA suppression Genome surveillance C. elegans germline RNAi Transposon Rutgers, The State University of New Jersey http...

... tissue. More recently, large collections of RNAi lines or cDNAs expressed under Gal4/ UAS control have been used to alter gene expression uniformly in specific tissues. However, these newer approaches are not easily combined with the efficient generation of genetic mosaics. The CoinFLP system described...

... interference (RNAi), was performed in pre-migratory FNC cells. Soon after electroporation of RNAi molecules, Smad1 inactivation abolished the expression of Foxg1 in the chick telencephalon, resulting in dramatic microcephaly and partial holoprosencephaly. In addition, the depletion of Foxg1 activity altered...

... to megagametogenesis. ARGONAUTE5 ( AGO5 ), a putative effector of small RNA (sRNA) silencing pathways, was found to be expressed around reproductive cells during megasporogenesis, and a novel semi-dominant ago5-4 insertion allele showed defects in the initiation of megagametogenesis. Expression of a viral RNAi...

... about how cell numbers are controlled in specific cell cohorts along the dorsoventral axis of the neural tube. Here, we show that FatJ cadherin, identified in a large-scale RNA interference (RNAi) screen of cadherin genes expressed in the neural tube, is localised to progenitors in intermediate regions...

... Physcomitrella patens . Plant Physiol. 133 , 470 - 474 . Bezanilla M. , Perroud P. F. , Pan A. , Klueh P. , Quatrano R. S. ( 2005 ). An RNAi system in Physcomitrella patens with an internal marker for silencing allows for rapid identification of loss of function phenotypes...

... play a central role in establishing proper fates along the entire DV axis in this animal; laser ablation of midline cells causes a failure to form neurogenic ectoderm and Ph-sim RNAi results in severely dorsalized embryos lacking both neurogenic ectoderm and the appendage-bearing lateral ectoderm...

... of the animals and repression of aboral structures (i.e. stolons). The expression of Wnt3, Tcf and Brachyury was upregulated and became ubiquitous. Rescue experiments using Tcf RNAi resulted in normal metamorphosis and quantitatively normal Wnt3 and Brachyury expression. Isolated, decapitated polyps regenerated...

... and invertebrate tissues ( Briscoe et al., 2001 ; Dessaud et al., 2008 ; Ingham and McMahon, 2001 ; Ingham and Placzek, 2006 ). The predominance of central gene expression in the severely affected At-smo RNAi germ disc prior to stage 5 suggests that At-Hh initially travels from its source (the outside and rim...

...Jianwu Bai; Richard Binari; Jian-Quan Ni; Marina Vijayakanthan; Hong-Sheng Li; Norbert Perrimon To facilitate the genetic analysis of muscle assembly and maintenance, we have developed a method for efficient RNA interference (RNAi) in Drosophila primary cells using double-stranded RNAs (dsRNAs...

.... In addition, DEPS-1 is required for RNA interference(RNAi) of germline-expressed genes, possibly because DEPS-1 promotes the accumulation of RDE-4, a dsRNA-binding protein required for RNAi. A genome wide analysis of gene expression in deps-1 mutant germ lines identified additional targets of DEPS-1...

... for correspondence (e-mail: [email protected] ) 13 6 2006 © 2006. 2006 siRNA RNAi Heterochrony Arabidopsis Shoot growth is characterized by the repeated production of lateral organs from the shoot apical meristem. The nature of these organs varies over time,undergoing both...

[email protected] and [email protected] ) 10 4 2006 © 2006. 2006 Spider Embryogenesis dpp sog Antagonist Body axis formation RNAi The fertilized eggs of Xenopus , as well as those of many other vertebrates, are transformed from a radially symmetrical form...

... is poorly understood. In C. elegans , the body wall muscles (BWMs)also have plasma membrane extensions called muscle arms that are guided to the motor axons where they form the postsynaptic element of the NMJ. To investigate the regulation of muscle membrane extension, we screened 871 genes by RNAi...

... and identified distinct classes of upregulated genes during this process. Fifty-seven genes were then tested functionally by RNAi. We found six genes affecting various aspects of cellular architecture: membrane growth, organelle transport or organisation and junction assembly. We focus here on charleston ( char...

... Embryo Motoneurons RNAi Optical projection tomography The T-box transcription factors are a family of developmentally regulated DNA-binding proteins that play important roles in organogenesis( Papaioannou, 2001 ). Their function is conserved throughout evolution, and they participate...

... insect Oncopeltus fasciatus . We find that in Oncopeltus, even-skipped striped expression initiates in a segmental and not pair-rule pattern. Weak RNAi suppression of Oncopeltus even-skipped shows no apparent pair-rule like phenotype, while stronger RNAi suppression shows deletion of nearly the entire...

.... and Klingler, M. ( 2004 ). Divergent segmentation mechanism in the short germ insect Tribolium revealed by giant expression and function. Development 131 , 1729 -1740. Bucher, G., Scholten, J. and Klingler, M. ( 2002 ). Parental RNAi in Tribolium (Coleoptera). Curr. Biol. 12 , R85 -R86...

... RNA interference (RNAi), we demonstrate that gene silencing of DjPum dramatically reduces the number of neoblasts, thus supporting the intriguing hypothesis that stem cell maintenance may be an ancestral function of PUF proteins. † Author for correspondence (e-mail: [email protected]...