... interests The authors declare no competing or financial interests. 24 8 2023 9 7 2024 © 2024. Published by The Company of Biologists Ltd 2024 https://www.biologists.com/user-licence-1-1/ Summary: Combinatorial multi-omics analyses using ATAC-seq, single-cell RNA-seq...

... nanoproteomics uncovers the relationship between RNAs and proteins during Arabidopsis thaliana early embryogenesis. Arabidopsis thaliana Proteomics Early embryogenesis RNA-seq Shenzhen Municipal Science and Technology Innovation Council 20200925153547003 State Key Laboratory...

... encompasses many major morphological events, including segmentation, body regionalization, gut morphogenesis and limb development. In addition, we use short- and long-read RNA-seq to generate an improved Parhyale genome annotation, enabling deeper classification of identified regulatory elements. We discover...

... development. Selfing Isogenic Forward genetics Tail bud Noto Mesogenin Mutant RNA-seq University of Basrah http://dx.doi.org/10.13039/100012026 National Institute of Child Health and Human Development http://dx.doi.org/10.13039/100000071 R15HD060017 Valdosta...

...: The transcriptional targets of SMAD4 in early limb buds are identified and the largely opposing impact of BMP and SHH signaling on early digit patterning and outgrowth is revealed. SMAD4 BMP SHH Limb development Anterior Mouse ChIP-seq RNA-seq Cistrome Schweizerischer Nationalfonds zur...

... Maternal deposition RNA-seq Transcript regulation Xenopus National Institutes of Health 100000002 R01GM42314 R35GM127069 National Institute of Mental Health 100000025 1U01MH115747-01A1 Overlook International Foundation The FET family of atypical RNA...

... analyze the transcriptome of discrete but continuously differentiating germline cysts. We first isolated single cysts at each recognizable stage from wild-type testes, which were subsequently applied for RNA-seq analyses. Our data delineate a high-resolution transcriptome atlas in the entire male GSC...

... in different regions of the central nervous system. Whether astrocyte diversity is attributable to developmental processes and bears functional consequences, especially in humans, is unknown. RNA-seq of human pluripotent stem cell-derived regional astrocytes revealed distinct transcript profiles, suggesting...

... in inner ear development and function. Otocyst Conditional mutant Ligand trap RNA-Seq National Institutes of Health 10.13039/100000002 R01 DC011819 R01 DC004185 National Science Foundation 10.13039/100000001 IOS-1239422 The membranous labyrinth of the mammalian...

... RNA with 4-thiouracil in a specific cell type in vivo followed by detection through an RNA-seq-based method that specifically distinguishes the thiolated uridine by base conversion. This method has successfully identified the cell type-specific transcriptome in three different tissues: endothelial...

... Amniotes Early embryo RNA-seq Signaling pathways National Research Foundation of Korea 10.13039/501100003725 2015R1A3A2033826 Avian species have been used as valuable research models for virology, cancer, immunology, developmental biology and biotechnology ( Streit et al., 2000...

... of the Creative Commons Attribution License ( http://creativecommons.org/licenses/by/3.0 ), which permits unrestricted use, distribution and reproduction in any medium provided that the original work is properly attributed. Highlighted Article: A comprehensive analysis of single-cell RNA-seq data from...

... manipulation of Xenopus laevis PGCs reveal novel roles for sox7 and pou5f3.3 in PGC development and a high degree of conservation between the Xenopus and human PGC transcriptomes.  Xenopus RNA-seq PGCs sox7 pou5f3.3 ( oct60 ) Germ plasm National Institutes of Health 10.13039...

...Mike Adam; Andrew S. Potter; S. Steven Potter Single-cell RNA-seq is a powerful technique. Nevertheless, there are important limitations, including the technical challenges of breaking down an organ or tissue into a single-cell suspension. Invariably, this has required enzymatic incubation at 37°C...

... as they are clustered at the midline, resembling other midline structures important for maintaining the bilateral organization of the neural tube. Adhesion Cell movement Midline Pituitary Chick RNA-seq Sp8 Placodes are discrete thickenings of the cranial ectoderm localized in the proximity...

... of heart development, during which many congenital heart disease malformations likely arise, we conducted an RNA-seq timecourse in zebrafish from 30 hpf to 72 hpf and identified 5861 genes with altered expression. We clustered the genes by temporal expression pattern, identified transcription factor...

... strains, we performed an RNA-seq screen, identifying tip- and stalk-enriched gene sets in the developing collecting duct system. Detailed in situ hybridization studies of tip-enriched predictions identified UBT-enriched gene sets conserved between the mouse and human kidney. Comparative spatial analysis...

... in the genome is poorly understood. Here we identified the transcriptomes of Xenopus foregut and hindgut progenitors, which are conserved with mammals. Using RNA-seq and ChIP-seq we show that BMP/Smad1 regulates dorsal-ventral gene expression in both the endoderm and mesoderm, whereas Wnt/β-catenin acts...

.../ Summary: RNA-sequencing analysis identifies 90 novel mRNAs that are enriched at the vegetal pole of Xenopus laevis oocytes, and reveals novel roles for some of these mRNAs in primordial germ cell development. Xenopus RNA-seq PGCs Localized RNAs Gene pathways Scaffold For many...

... sequencing has been used to study developmental and stem cell biology, providing insights into cell type diversity and developmental dynamics. RNA-Seq Computational biology Gene regulatory networks Pseudotime Single cell Stem cells To characterize the diversity of cell types...