... by the RNA-binding protein Rbp4 and its partner Fest. Here, we show that the spermatocyte-specific protein Lut is required for translational repression of cycB in an 8-h window just before spermatocytes are fully mature. In males mutant for rbp4 or lut , spermatocytes enter and exit meiotic division 6-8 h...

...Laura Thomas; Andrea Putnam; Andrew Folkmann ABSTRACT A hallmark of all germ cells is the presence of germ granules: assemblies of proteins and RNA that lack a delineating membrane and are proposed to form via condensation. Germ granules across organisms share several conserved components...

... of the distinct molecular mechanisms modulating BAF chromatin binding, including its combinatory assemblies, DNA/histone modification-binding modules and post-translational modifications, as well as its interactions with proteins, RNA and lipids. This Review aims to serve as a primer for future studies to decode...

... mammals, urges a rethinking of the nature of hereditary information carriers and the ways in which they encode heritable traits. In this short opinion piece, we discuss how potential hereditary information carriers such as DNA-associated proteins, epigenetic marks, RNAs and organelles could function via...

...David Israeli; Ronit Nir; Talila Volk Regulation of RNA metabolism plays a major role in controlling gene expression during developmental processes. The Drosophila RNA-binding protein Held out wing (HOW), regulates an array of developmental processes in embryonic and adult growth. We have...

...Sherri Evans Bergsten; Tao Huang; Seema Chatterjee; Elizabeth R. Gavis ABSTRACT Localization of nanos ( nos ) mRNA to the germ plasm at the posterior pole of the Drosophila embryo is essential to activate nos translation and thereby generate abdominal segments. nos RNA localization is mediated...

...Jian Zhang; Mary Lou King ABSTRACT An RNA localized to the vegetal cortex of Xenopus oocytes encodes a novel T-box protein (VegT) capable of inducing either dorsal or posterior ventral mesoderm at different times in development. VegT is a nuclear protein and its C-terminal domain can activate...

... proteins requires the presence of functional products from all four of the msl genes, the absence of MSL-2 in the female would prevent X chromosome binding of the other three MSLs and in so doing would presumably prevent hypertranscription of the female’s X chromosomes. SXL is an RNA-binding...

... (SNuPE) assay, we found that the amount of Xist RNA in adult kidney of three mouse strains was less than ∼2000 transcripts per cell, with only modest differences between strains carrying different Xce alleles. Female embryos 7.5 days post coitum had the same number of Xist transcripts per cell...

... methods to examine expression of the murine Fgf-5 gene during embryogenesis. Fgf-5 RNA was detected at seven distinct sites in the developing mouse embryo: (1) postimplantation epiblast (embryonic day , (2) lateral splanchnic mesoderm , (3) lateral somatic mesoderm , (4) myotomes , (5) mastication muscle...

...Irene E. Schauer; William B. Wood We have developed a nucleotide incorporation assay for run-on transcription in C. elegans embryonic extracts as an approach to characterizing early transcription. The incorporation is primarily polymerase II-catalyzed RNA synthesis, producing transcripts...

... below, we focus on how these data bear on our current understanding of the establishment of early regional specializations in the ectoderm. Early regional specialization in embryonic ectoderm CKsel RNA serves as a marker that allows for the direct assessment of a regional specialization event in early...

... if the ex­pression of any gene product is sensitive to ECM disruption, a differential cDNA screen compared poly (A + ) RNA from BAPN-arrested and control embryos in Lytechinus. A cDNA clone was isolated from this screen that represented a 2Ί kb mRNA that did not accumulate during BAPN treatment. Removal of BAPN...

...A. Whyte; H. J. Stewart ABSTRACT Blastocyst material was obtained from 25 pigs during the period 10 to 33 days post coitum , and fixed thin sections of tissue were hybridized in situ to sense and antisense fos RNA probes synthesized using the expression vector Bluescribe M13 + . Indirect...

...Iain W. Mattaj; Jörg Hamm 10 11 1988 © 1989 by Company of Biologists 1989 RNA splicing mechanism endogenous gene U snRNP ribonucleoprotein Xenopus mouse Analysis of the splicing of several different genes has led to the discovery of RNA-processing events...

...Jacques Hourdry; Annie Brulfert; Michel Gusse; Damien Schoevaert; Michael V. Taylor; Marcel Mechali The expression of the proto-oncogene c-myc during oogenesis and embryonic development was followed by in situ hybridization using a cytological protocol adapted to amphibian embryos. The c-myc RNA...

... segments. Such embryos can be ‘rescued’, i.e. restored to normal development, by microinjection of cytoplasm or RNA from unirradiated donor embryos. Most of the rescued embryos look completely normal and many of them hatch spontaneously. The rescuing activity decreases from the anterior to the posterior...

.... that encoding the Nerve Growth Factor receptor, is strongly activated by the withdrawal of axons, and that this activation is largely independent of cAMP. 09 12 1987 © 1988 by Company of Biologists 1988 myelination Schwann cells gene expression cyclic AMP NGF RNA We thank Drs...

... synthesized RNA, respectively. The label was localized in different structural components of developing nucleoli using electron microscopic autoradiography. Careful study of the relationship between the structural pattern and nucleic acid distribution made it possible to define four stages of embryonic...

...C. R. Kintner; D. A. Melton ABSTRACT We have isolated Xenopus laevis N-CAM cDNA clones and used these to study the expression of N-CAM RNA during neural induction. The results show that the first marked increase in N-CAM RNA levels occurs during gastrulation when mesoderm comes in contact...