...Elisabeth C. Kugler; James Frost; Vishmi Silva; Karen Plant; Karishma Chhabria; Tim J. A. Chico; Paul A. Armitage ABSTRACT Zebrafish transgenic lines and light sheet fluorescence microscopy allow in-depth insights into three-dimensional vascular development in vivo . However, quantification...

... segmentation step is based on the bright field image of the organoid ( Fig. 1 B), followed by a quantification step that takes into account all bright field and fluorescence channels available ( Fig. 1 C). The rationale behind this design is that bright field images are usually readily available when...

... ). At the present time, developmental stages of mammalian embryos are either defined by gestational time, by a suitable staging system (such as the Theiler stages) or by somite counting where possible. Limb development Limb staging Morphometrics Quantification Staging system Web application © 2011...