... expressing proneural genes, including ptf1a , neurog1 and atoh1a/b/c . Studies in mammals previously suggested that these genes determine cerebellar neuron cell fate. However, our studies on ptf1a;neurog1 zebrafish mutants and lineage tracing of ptf1a -expressing progenitors have revealed that the ptf1a...

... are derived from neural progenitors expressing the proneural gene ptf1a . In this study, we found that the homeobox gene gsx2 was co-expressed with ptf1a in IO progenitors in zebrafish. Both gsx2 and ptf1a zebrafish mutants showed a strong reduction or loss of IO neurons. The expression of ptf1a...

... systems, Dre/RoxP and Cre/LoxP, to independently trace green fluorescent Pdx1-lineage cells and red fluorescent Ptf1a-lineage cells in the developing and adult mouse pancreas. We detected a few Pdx1 + /Ptf1a − lineage cells in addition to the vast majority of Pdx1 + /Ptf1a + lineage cells in the pancreas...

... of a presumed pancreatic-commitment transcription factor, Ptf1a, in embryonic mouse endoderm (Ptf1a EDD ) dramatically expanded the pancreatic gene regulatory network within the foregut. Ptf1a EDD temporarily suppressed Sox2 broadly over the anterior endoderm. Pancreas-proximal organ territories underwent full...

... ). Consistent with this dynamic definition of the rhombic lip as an inductive interface, the lineage boundaries between the ventricular zone, rhombic lip and roof plate are somewhat blurred ( Fig. 5 ). The ventricular zone of the cerebellum is characterised by Ptf1a expression ( Fig. 5 A,B) and gives rise...

...-helix transcription factors (TFs), Ascl1 and Ptf1a, have contrasting functions in specifying these neurons. To understand how Ascl1 and Ptf1a function in this process, we identified their direct transcriptional targets genome-wide in the embryonic mouse neural tube using ChIP-Seq and RNA-Seq. We show...

... expression of Pdx1 and Ptf1a/p48 results in the stable conversion of posterior endoderm into endocrine and exocrine pancreatic tissue . Genes Dev. 20 , 1441 – 1446 . Apelqvist A. , Li H. , Sommer L. , Beatus P. , Anderson D. J. , Honjo T. , de Angelis M. H...

... and thereby represses Neurog3 and endocrine differentiation in neighboring cells by lateral inhibition. Here we show in mouse that Dll1 and Hes1 expression deviate during regionalization of early endoderm, and later during early pancreas morphogenesis. At that time, Ptf1a activates Dll1 in multipotent...

... exocrine tissue. * Author for correspondence (e-mail: [email protected] ) 22 8 2007 © 2007. 2007 Zebrafish Pancreas FGF Signaling Endoderm Mesoderm ptf1a In amniotes, the pancreas originates from the thickening of the endodermal epithelium leading...

... expressions, which are influenced by pattern formation and cell-specification pathways. We report here that the tissue-specific bHLH transcription factor Ptf1a (also known as PTF1-p48 ) is expressed from embryonic day 12.5 of gestation (E12.5)to postnatal day 3 (P3) during retinogenesis in the mouse. Using...

... and one class of glial cells, all derived from a population of multipotent progenitors. There is little information on the molecular mechanisms governing the specification of cell type identity from multipotent progenitors in the developing retina. We report that Ptf1a, a basic-helix-loop-helix (bHLH...

... does not require aorta or endothelial cell interactions, but dorsal pancreatic bud emergence and the maintenance of Pdx1 expression does. Aortal endothelial cells induce the crucial pancreatic transcription factor Ptf1a in the dorsal pancreatic endoderm; whereas the vitelline veins, which are normally...