... subcellular protein synthesis events in real time in vivo . This Protein Translation Reporting (PTR) technique uses a genetic tag that produces a stoichiometric ratio of a small peptide portion of a split fluorescent protein and the protein of interest during protein synthesis. We show that the split...

... the phosphorylation of AKT1. Thus, our studies reveal the function of Axin1 in regulating protein synthesis, beyond its role as a Wnt signaling regulator. In the present study, it was tentatively inferred by analyses of our sequencing results that Axin1 negatively regulates ribosomal biogenesis. Ribosomal...

... transcriptome of MG. Of these genes, those that are induced are biased towards regulation of ribosome biogenesis, protein synthesis, DNA synthesis, and cell division, which are the top cellular processes affected by retinal injury, suggesting that Mycb and Mych are potent MG reprogramming factors. Consistent...

.... These methods can enable quantitative exploration of protein synthesis and degradation mechanisms that contribute to developmental cell fate specification. Furthermore, they may support functional analysis of protein conformations and activities in single cells, and thus link protein functions to developmental...

... double knockout (dKO) mice display neural tube defects (NTDs) coupled with reduced proliferation and precocious differentiation of NPCs. Using ribosomal protein 24 hypomorphic mice ( Rpl24 Bst/+ ) as a genetic tool to dampen global protein synthesis, we found that Lin28a −/− ;Rpl24 Bst/+ compound mutants...

... not abolish, embryonic and adult myogenesis. Raptor Rictor Protein synthesis Satellite cells Muscle regeneration Rapamycin Canton of Basel-Stadt Canton of Basel-Landschaft Schweizerischer Nationalfonds zur Förderung der Wissenschaftlichen Forschung 10.13039...

... ( bonsaï ) in a homolog of the prokaryotic ribosomal protein, RPS15, causes a growth defect that is non-cell-autonomous. Our results emphasize the importance of translational regulation for the exit from quiescence. They suggest that the level of protein synthesis required for cell cycle progression varies...

...S. K. Rehen; M. H. Varella; F. G. Freitas; M. O. Moraes; R. Linden ABSTRACT The role of protein synthesis in apoptosis was investigated in the retina of developing rats. In the neonatal retina, a ganglion cell layer, containing neurons with long, centrally projecting axons, is separated from...

... synthesis in a mid blastula, but has become independent of it by the late blastula stage. In animal caps induced to become mesoderm, the time when protein synthesis is required for subsequent elongation immediately follows the time of induction, and is not related to developmental stage. For elongation...

... by comparing protein synthesis in normal and UV-irradiated embryos. About 5% of 433 labelled polypeptides detected in 2D gels were affected by UV irradiation. The most prominent protein is a 30 kDa cytoskeletal component (p30), whose synthesis is abolished by UV irradiation. p30 synthesis peaks during...

.... Several specific alterations in the protein synthesis pattern of DBA/2 androgenones were observed that reflect a defect in the regulatory mechanisms that normally modulate the synthesis of these proteins between the 8-cell and blastocyst stages. These results are consistent with a model in which...

... occurs that is largely complete by 15 h following the first cleavage. Once this reprogramming is complete, very little change is observed during the late 2-cell and 4-cell stages. Cluster analysis of individual protein synthesis patterns reveals a limited number of coordinately regulated protein sets...

... an in vivo assay to determine if the availability of mRNA and/or components of the translational machinery are limiting protein synthesis in the unfertilized egg. This assay involves the use of a protein synthesis elongation inhibitor to create a situation in the egg in which there is excess translational...

... the major series of changes in protein synthesis that occurs after entry into a metaphase. We conclude therefore that the mixing of the nucleoplasm and cytoplasm is not essential for the initiation or progression of the protein repro-gramming process during maturation. In addition, the experiments show...

... of newly synthesised proteins by [ 35 S]methio-nine radiolabelling of oocytes demonstrated that ODN injection can trigger a general decrease in both label uptake and protein synthesis. Qualitative effects on protein synthesis could also be observed, particularly a decrease in synthesis of high molecular...

...J. R. Collier ABSTRACT Two-dimensional electrophoresis of labeled proteins and hybridization of mRNAs with specific gene probes was used to demonstrate changes in protein synthesis, and the cytoplasmic control of actin maternal mRNAs during the early development of llyanassa . The isolated polar...

...I. Néant; P. Guerrier The dependency of some key events of the cell cycle upon pH, phosphorylation and protein synthesis was investigated during meiosis reinitiation of Patella vulgata oocytes stimulated by ammonia. In this report, we show, through heterologous microinjection experiments...

... and continued helper role of the resident pronucleus for the participation by an 8-cell nucleus in reconstituted eggs. 04 09 1987 © 1987 by Company of Biologists 1987 mouse embryo nuclear transfers protein synthesis cytoplasmic interaction transplantation Nuclear transplantation...

... is that of a ‘Born’s crescent’ with a conspicuous ‘vitelline wall’ ( Pasteels, 1964 ). When oocytes are enucleated before maturation, HS has no effect on GC formation. A nuclear factor is therefore essential, as has been demonstrated in early GC formation induced by inhibitors of protein synthesis. Finally...

...S. Cascio; J. B. Gurdon ABSTRACT The incubation of Xenopus embryo fragments in cycloheximide at 5 or 10μgml −1 rapidly inhibits protein synthesis to 10 % or less of control levels. In most batches of embryos, treatment with cycloheximide for up to 1 h causes no obvious cellular damage and protein...