... the formation of actin-rich long cellular extensions that are important for the delamination and the migration of the invasive cluster. We show that IIS specifically activates the expression of the actin regulator chickadee , the Drosophila homolog of Profilin, which is essential for promoting the formation...

... ( chic ), the fly ortholog of Profilin, mediates soma-germline interactions. Knockdown of Chic in the soma results in sterility and severely disrupted spermatogenesis due to defective encapsulation. To study this defect further, we developed a permeability assay to analyze whether the germline...

... controlled cytoskeletal remodeling, and we show that SC lamellipodia formation depends on the function of profilin 1 (Pfn1), an actin-binding protein involved in microfilament polymerization. Pfn1 is inhibited upon phosphorylation by ROCK, a downstream effector of the integrin linked kinase pathway. Thus...

... to the apical hub and somatic cyst stem cell (CySC) identity. Here, we demonstrate that chickadee , the Drosophila gene that encodes profilin, is required cell autonomously to maintain GSCs, possibly facilitating localization or maintenance of E-cadherin to the GSC-hub cell interface. Germline specific...

... interacted with endogenous Profilin1 ( Fig. 1C ). Additionally the FH1-but not the FH2-containing construct of Daam1 was found to interact with endogenous Profilin1 ( Fig. 1C ). Fig. 1. Profilin binds to the FH1 domain of Daam1. ( A ) Schematic diagram of the Daam1 and Profilin1 constructs showing...

... to that which occurs prematurely in cappuccino and spire mutant oocytes. After examining a number of mutants that affect the actin cytoskeleton, we have found that chickadee , which encodes the actin-binding protein, profilin, shares this phenotype. In addition to the microtubule misregulation, mutants...

...Esther M. Verheyen; Lynn Cooley ABSTRACT The chickadee gene of Drosophila encodes profilin, a small actin binding protein. We present the first analysis of the effects of profilin deletion in a multicellular organism. Genomic deletions of the chickadee locus result in a late embryonic lethal...