...Lacy J. Barton; Lorena Roa-de la Cruz; Ruth Lehmann; Benjamin Lin ABSTRACT The germline provides the genetic and non-genetic information that passes from one generation to the next. Given this important role in species propagation, egg and sperm precursors, called primordial germ cells (PGCs...

.... Male germ cell development starts when pluripotent cells undergo specification to sexually uncommitted primordial germ cells, which act as precursors of both oocytes and spermatozoa. Male-specific development subsequently occurs in the fetal testes, resulting in the formation of spermatogonial stem...

...-1746891 National Institutes of Health http://dx.doi.org/10.13039/100000002 R37HD037047 T32 GM007445 Germ plasm P-bodies Germline Primordial germ cells RNP granules C. elegans Highlighted Article: This paper describes a new condensate in the C. elegans germ plasm...

...Yuki Naitou; Go Nagamatsu; Nobuhiko Hamazaki; Kenjiro Shirane; Masafumi Hayashi; Makoto Hayashi; Satoru Kobayashi; Katsuhiko Hayashi ABSTRACT In mammals, primordial germ cells (PGCs), the origin of the germ line, are specified from the epiblast at the posterior region where gastrulation...

... of cytoplasm, termed the rachis. How germ cells undergo cytokinesis while maintaining this syncytial architecture is not completely understood. Here, we use live imaging to characterize primordial germ cell (PGC) division in C. elegans first-stage larvae. We show that each PGC possesses a stable intercellular...

...Luca Giovanni Di Giovannantonio; Dario Acampora; Daniela Omodei; Vincenzo Nigro; Pasquale Barba; Elisa Barbieri; Ian Chambers; Antonio Simeone ABSTRACT In mammals, the pre-gastrula proximal epiblast gives rise to primordial germ cells (PGCs) or somatic precursors in response to BMP4 and WNT...

...Grace V. Hancock; Sissy E. Wamaitha; Lior Peretz; Amander T. Clark ABSTRACT The peri-implantation window of mammalian development is the crucial window for primordial germ cell (PGC) specification. Whereas pre-implantation dynamics are relatively conserved between species, the implantation window...

...Tzofia Bialistoky; Diane Manry; Peyton Smith; Christopher Ng; Yunah Kim; Sol Zamir; Victoria Moyal; Rachel Kalifa; Paul Schedl; Offer Gerlitz; Girish Deshpande ABSTRACT During embryonic gonad coalescence, primordial germ cells (PGCs) follow a carefully choreographed migratory route circumscribed...

..., OCT4 and PRDM14, in mouse embryonic stem cells, epiblast-like cells and primordial germ cell-like cells (PGCLCs). PGCLCs are an important system for elucidating primordial germ cell development in mice. We monitored PRDM14 binding during the specification of PGCLCs, identifying direct targets of PRDM14...

...Toshihiro Kobayashi; M. Azim Surani ABSTRACT In mice, primordial germ cells (PGCs), the precursors of eggs and sperm, originate from pregastrulation postimplantation embryos. By contrast, the origin of human PGCs (hPGCs) has been less clear and has been difficult to study because of the technical...

...Nathalie Oulhen; S. Zachary Swartz; Jessica Laird; Alexandra Mascaro; Gary M. Wessel Stem cells in animals often exhibit a slow cell cycle and/or low transcriptional activity referred to as quiescence. Here, we report that the translational activity in the primordial germ cells (PGCs) of the sea...

... phosphatases known to regulate the directional migration of primordial germ cells (PGCs). PGC and CVM cell types interact while PGCs are en route to the somatic gonadal mesoderm, and previous studies have shown that CVM impacts PGC migration. In turn, we found here that CVM cells exhibit an affinity for PGCs...

... efficient CRISPR mutagenesis with transplantation of mutated primordial germ cells into a wild-type host. Tested using Xenopus tropicalis , we show that founders containing transplants transmit mutant alleles with high efficiency. F1 offspring from intercrosses between F0 animals that carry embryonic lethal...

...Girish Deshpande; Diane Manry; Nicholas Jourjine; Vladic Mogila; Henny Mozes; Tzofia Bialistoky; Offer Gerlitz; Paul Schedl Coalescence of the embryonic gonad in Drosophila melanogaster requires directed migration of primordial germ cells (PGCs) towards somatic gonadal precursor cells (SGPs...

...Rana Mainpal; Jeremy Nance; Judith L. Yanowitz Despite the central importance of germ cells for transmission of genetic material, our understanding of the molecular programs that control primordial germ cell (PGC) specification and differentiation are limited. Here, we present findings that X...

...Erna Magnúsdóttir; M. Azim Surani Primordial germ cells (PGCs) are the precursors of sperm and eggs, which generate a new organism that is capable of creating endless new generations through germ cells. PGCs are specified during early mammalian postimplantation development, and are uniquely...

... of epimutations. Notably, primordial germ cells (PGCs) erase genome-wide DNA methylation and H3K9 dimethylation marks in a stepwise manner during migration and gonadal periods. In this study, we profiled DNA and histone methylation on transposable elements during PGC development, and examined the role of DNA...

.... Furthermore, primordial germ cells express, and appear to be functionally dependent upon, transcription factors that characterise the pluripotent state. We hypothesise that germ cells require pluripotency factors to control a de-restricted epigenome. Consequently, they harbour latent potential, as manifested...

...Jessica R. K. Seifert; Ruth Lehmann Trans-epithelial migration describes the ability of migrating cells to cross epithelial tissues and occurs during development, infection, inflammation, immune surveillance, wound healing and cancer metastasis. Here we investigate Drosophila primordial germ cells...

...Mitinori Saitou; Saya Kagiwada; Kazuki Kurimoto Epigenetic modifications are crucial for the identity and stability of cells, and, when aberrant, can lead to disease. During mouse development, the genome-wide epigenetic states of pre-implantation embryos and primordial germ cells (PGCs) undergo...