... signaling molecules, transcription factors and epigenetic modifiers. Spermatogenesis Meiosis Spermiogenesis Primordial germ cell Gonocyte Spermatogonium Spermatogonial stem cell Spermatocyte Spermatid Chinese STI 2030-Major Projects 2023ZD040750305 National Natural...

...Christopher B. Cooke; Christopher Barrington; Peter Baillie-Benson; Jennifer Nichols; Naomi Moris ABSTRACT Primordial germ cells (PGCs) are the early embryonic precursors of gametes – sperm and egg cells. PGC-like cells (PGCLCs) can currently be derived in vitro from pluripotent cells exposed...

...; however, it is less clear how niche BM relays signals to stem cells that it does not contact. Here, we examine how niche BM regulates Caenorhabditis elegans primordial germ cells (PGCs). BM regulates PGC quiescence even though PGCs are enwrapped by somatic niche cells and do not contact the BM; this can...

... that the original work is properly attributed. Summary: A survey of germ granules across organisms and developmental stages, highlighting emerging themes regarding granule regulation, dynamics and proposed functions. RNA Condensate Germ granule Oocyte Primordial germ cell Sperm National...

...Christopher B. Cooke; Naomi Moris ABSTRACT Primordial germ cells (PGCs) form early in embryo development and are crucial precursors to functioning gamete cells. Considerable research has focussed on identifying the transcriptional characteristics and signalling pathway requirements that confer PGC...

...? Integrating findings from diverse species, we conclude that the final commitment of the germline to gametogenesis – the process of germ cell determination – occurs after primordial germ cells (PGCs) colonize the gonads. Combining this understanding with medical findings, we present a model whereby germ cell...

...Stefan Redl; Antonio M. de Jesus Domingues; Edoardo Caspani; Stefanie Möckel; Willi Salvenmoser; Maria Mendez-Lago; René F. Ketting ABSTRACT Primordial germ cells (PGCs) are the precursors of germ cells, which migrate to the genital ridge during early development. Relatively little is known about...

...Toshihiro Kobayashi; Hisato Kobayashi; Teppei Goto; Tomoya Takashima; Mami Oikawa; Hiroki Ikeda; Reiko Terada; Fumika Yoshida; Makoto Sanbo; Hiromitsu Nakauchi; Kazuki Kurimoto; Masumi Hirabayashi ABSTRACT Primordial germ cells (PGCs), the founder cells of the germline, are specified in pre...

...Kentaro Mochizuki; Yukiko Tando; Tamotsu Sekinaka; Kei Otsuka; Yohei Hayashi; Hisato Kobayashi; Asuka Kamio; Yumi Ito-Matsuoka; Asuka Takehara; Tomohiro Kono; Noriko Osumi; Yasuhisa Matsui ABSTRACT In mouse embryos, primordial germ cells (PGCs) are fate-determined from epiblast cells. Signaling...

... including: inhibition of specific transcription, degradation of specific mRNAs and inhibition of tissue morphogenesis. These results document a signaling mechanism required for the sequential restriction of germ cell factors, which causes a specific set of embryonic cells to become the primordial germ cells...

...Lorna Taylor; Daniel F. Carlson; Sunil Nandi; Adrian Sherman; Scott C. Fahrenkrug; Michael J. McGrew In this work we use TALE nucleases (TALENs) to target a reporter construct to the DDX4 ( vasa ) locus in chicken primordial germ cells (PGCs). Vasa is a key germ cell determinant in many animal...

... of germ cells in mice. Our functional analyses of the Gryllus Blimp-1 ortholog revealed that it is essential for Gryllus primordial germ cell development, and is regulated by upstream input from the BMP signaling pathway. This functional conservation of the epistatic relationship between BMP signaling...

.... The ‘last cell standing’ model describes primordial germ cell (PGC) development in axolotls, in which PGCs are maintained by an extracellular signalling niche, and germ line commitment occurs after gastrulation. Here, we propose that this ‘stochastic’ mode of PGC specification is conserved in vertebrates...

..., in Xenopus and zebrafish embryos primordial germ cells (PGCs) are specified by germ plasm that is inherited from the egg; in mice, Blimp1 expression in the epiblast mediates the commitment of cells to the germ line. How these disparate mechanisms of PGC specification evolved is unknown. Here, in order...

...Shinpei Yamaguchi; Kazuki Kurimoto; Yukihiro Yabuta; Hiroyuki Sasaki; Norio Nakatsuji; Mitinori Saitou; Takashi Tada The pluripotency factor Nanog is expressed in peri-implantation embryos and primordial germ cells (PGCs). Nanog-deficient mouse embryos die soon after implantation. To explore...

...Ying Gu; Chris Runyan; Amanda Shoemaker; Azim Surani; Chris Wylie Steel factor is an essential survival and proliferation factor for primordial germ cells (PGCs) during their migration in the early mouse embryo. PGCs arise during gastrulation, and migrate into the posterior endoderm that becomes...

... in the mouse embryo commences in the primordial germ cells(PGCs) just after their formation, and a loss of this protein results in the germ cell-less phenotype in both sexes. By contrast, Nanos2 expression begins only in male PGCs after their entry into the genital ridge and a loss of this protein results...

...Miho Asaoka; Haifan Lin A fundamental yet unexplored question in stem cell biology is how the fate of tissue stem cells is initially determined during development. In Drosophila, germline stem cells (GSCs) descend from a subset of primordial germ cells (PGCs) at the onset of oogenesis. GSC...

..., the mechanisms of germ cell specification in mice remain unknown. We located precursors to primordial germ cells (PGCs) within early embryos, and show here that cell-cell interaction among these precursors is required for germ cell specification. We found that the expression of a calcium-dependent cell adhesion...

...Tomomi Yoshimizu; Masuo Obinata; Yasuhisa Matsui ABSTRACT Primordial germ cells (PGCs) in mice have been recognized histologically as alkaline phosphatase (AP) activity-positive cells at 7.2 days post coitum (dpc) in the extra-embryonic mesoderm. However, mechanisms regulating PGC formation...