... ovulation, a process that is highly conserved from flies to mammals. The factors that regulate spatiotemporal expression of MMP2 in follicle cells remain unknown. Here, we demonstrate crucial roles for the ETS-family transcriptional activator Pointed (Pnt) and its endogenous repressor Yan in the regulation...

... midgut constriction defects in Kah mutants similar to those of pointed ( pnt ). ChIP and RNA-Seq data analysis defined a Kah target-binding site similar to that of Snail, and identified a set of common target genes putatively regulated by Kah and Pnt during midgut constriction. Taken together, we report...

... downregulation of Pax6 with either the NURF nucleosome remodeling complex or the Pointed transcription factor transforms the head epidermis into an antenna. This is a previously unidentified fate change that is also not observed with the loss of individual genes. We propose that the use of multi-gene knockdowns...

.... In Drosophila , the Ets protein Pointed P1 (PntP1) is required to generate INPs from type II neuroblasts. Here, we investigated how PntP1 promotes INP generation. By generating pntP1 -specific mutants and using RNAi knockdown, we show that the loss of PntP1 leads to both an increase in type II neuroblast number...

... positions, and their deployment of ramified arbors to cover specific neuropil territories to form a stereotyped astroglial map. Finally, we unraveled a molecular pathway for astrocyte differentiation in which the Ets protein Pointed and the Notch signaling pathway are required for astrogenesis; however...

...Arkadi Shwartz; Shaul Yogev; Eyal D. Schejter; Ben-Zion Shilo How signal transduction, which is dynamic and fluctuating by nature, is converted into a stable trancriptional response, is an unanswered question in developmental biology. Two ETS-domain transcription factors encoded by the pointed...

... that this enhancer requires the Egfr/Ras pathway transcription factor Pointed. Recently, others have shown that this same enhancer also requires the eye determining transcription factor Sine oculis(So). We discuss these data in terms of a model for a combinatorial code of furrow movement. § Author...

..., but not Rhomboid-2 or -4, and the neuregulin-like ligand Vein also function in survival signalling. Pointed mutants display the lowest frequency of fusions, suggesting that EGFR signalling may inhibit apoptosis primarily at the post-translational level. All ventral epidermal cells therefore require some level...

... activation, but does block nuclear transduction of Egfr activation through transcriptional repression of pointed , which encodes the nuclear effector of the pathway. Thus, Senseless promotes normal R8 differentiation by preventing the effects of autocrine stimulation by Spitz. An analogous relationship...

... propose that REPO plays a key role in both glial development and diversification. * Author for correspondence (e-mail: [email protected] ) 10 2 2003 © 2003. 2003 Drosophila Glia Neuron repo tramtrack pointed gcm The two major cell types in the nervous...

...Sebastian Granderath; Angelika Stollewerk; Steve Greig; Corey S. Goodman; Cahir J. O’Kane; Christian Klämbt In Drosophila , glial cell development depends on the gene glial cells missing ( gcm ). gcm activates the expression of other transcription factors such as POINTED and REPO, which control...

... formation of an embryonic midgut constriction at a precise location within the broad and uniform abdA expression domain. The constriction divides the abdA domain of the midgut into two chambers, the anterior one producing the Pointed (Pnt) ETS transcription factors and the posterior one the Odd-paired (Opa...

... lateral glial development is controlled by the gcm gene. Subsequent glial cell differentiation depends partly on the pointed gene. Here we describe a novel component required for all CNS glia development. The tramtrack gene encodes two zinc-finger proteins, one of which, ttkp69, is expressed in all non...

...-producing or midline cells, resulting in patterning in the dorsal follicle cell layer. We show here that Pointed, an ETS transcription factor, is required in dorsal follicle cells for this patterning. Loss of pointed results in the loss of midline cells and an excess of appendage-forming cells, a phenotype...

... and secondary target genes of DER, which mediate the determination of discrete ventral cell fates. High levels of DER activation in the ventralmost cells trigger expression of the transcription factors encoded by ventral nervous system defective ( vnd ) and pointed P1 ( pntP1 ). Concomitant with the induction...

... the glial cells recognize specific neuronal cells at the midline, they migrate posteriorly along their cell processes and thereby separate the segmental commissures. The gene pointed ( pnt ) is required for this glialneuronal cell interaction, as loss of function mutations lead to a change in the migration...