... anterior primitive streak progenitors to the notochord lineage. Gastrulation Human Micropatterns Notochord Pluripotent stem cells Biotechnology and Biological Sciences Research Council http://dx.doi.org/10.13039/501100000268 BB/W002310/1 BB/T017961/1 Wellcome Trust http...

... lineages. Although we have learned much from animal models, many details about human inner ear development remain elusive. We recently developed an in vitro model of human inner ear organogenesis using pluripotent stem cells in a 3D culture, fostering the growth of a sensorineural circuit, including hair...

...Olga G. Jaime; Jessica Arias; Shreya Pavani; April D. Pyle; Michael R. Hicks ABSTRACT The earliest skeletal muscle progenitor cells (SMPCs) derived from human pluripotent stem cells (hPSCs) are often identified by factors expressed by a diverse number of progenitors. An early transcriptional...

... that the middle cell layer could have pluripotent stem cell activity and its establishment requires the quiescent center-related SCR - SHR - WOX5 - PLT1 / 2 - JKD gene network. * These authors contributed equally to this work ‡ Author for correspondence ( [email protected] ) Handling Editor: Ykä...

... interactions Human development Disease modeling Pluripotent stem cells European Molecular Biology Organization http://dx.doi.org/10.13039/100004410 ALTF 321-2021 Wu Tsai Neurosciences Institute, Stanford University Stanford Bio-X New York Stem Cell Foundation...

...Daniel Medina-Cano; Emily K. Corrigan; Rachel A. Glenn; Mohammed T. Islam; Yuan Lin; Juliet Kim; Hyunwoo Cho; Thomas Vierbuchen ABSTRACT Directed differentiation of pluripotent stem cells (PSCs) is a powerful model system for deconstructing embryonic development. Although mice are the most advanced...

... analysis tools. Here, we introduce SyNPL: clonal pluripotent stem cell lines that employ optimised Synthetic Notch (SynNotch) technology to report cell-cell interactions between engineered ‘sender’ and ‘receiver’ cells in cultured pluripotent cells and chimaeric mouse embryos. A modular design makes...

... and human embryonic stem cells . Cell Stem Cell 6 , 215 - 226 . 10.1016/j.stem.2010.01.003 Hayashi , K. , Ohta , H. , Kurimoto , K. , Aramaki , S. and Saitou , M. ( 2011 ). Reconstitution of the mouse germ cell specification pathway in culture by pluripotent stem cells...

... remains elusive. Here, we found that human-specific heart brake lncRNA 1 ( HBL1 ) interacted with two PRC2 subunits, JARID2 and EED, in human pluripotent stem cells (hPSCs). Loss of JARID2, EED or HBL1 significantly enhanced cardiac differentiation from hPSCs. HBL1 depletion disrupted genome-wide PRC2...

... remains unclear. Here, we studied HOX clock pacing in human pluripotent stem cell-derived axial progenitors differentiating into diverse spinal cord motor neuron subtypes. We show that the progressive activation of caudal HOX genes is controlled by a dynamic increase in FGF signaling. Blocking the FGF...

... and regenerative medicine. Axis elongation Gastrulation Neuromesodermal progenitors Primitive streak Tail bud Pluripotent stem cells Lineage specification in the early vertebrate embryo is initiated during gastrulation (see Glossary, Box 1 ), which involves the formation of the three germ...

...Hideyuki Okano; Doug Sipp ABSTRACT Regenerative therapies, including both gene and cellular therapies, aim to induce regeneration of cells, tissues and organs and restore their functions. In this short Spotlight, we summarize the latest advances in cellular therapies using pluripotent stem cells...

... governing ethical considerations of human embryonic research have allowed us to enlighten this black box. Here, we will summarize the current knowledge of human XCI, mainly based on the analyses of embryos derived from in vitro fertilization and of pluripotent stem cells, and highlight any unanswered...

...Ana Luisa Rodrigues Toste de Carvalho; Alexandros Strikoudis; Hsiao-Yun Liu; Ya-Wen Chen; Tiago J. Dantas; Richard B. Vallee; Jorge Correia-Pinto; Hans-Willem Snoeck ABSTRACT Although strategies for directed differentiation of human pluripotent stem cells (hPSCs) into lung and airway have been...

...Jarmon G. Lees; David K. Gardner; Alexandra J. Harvey ABSTRACT As human pluripotent stem cells (hPSCs) exit pluripotency, they reportedly switch from glycolytic energy production to primarily mitochondrial metabolism. Here, we show that upon ectoderm differentiation to neural precursor cells (NPCs...

... is accompanied by progressive transcriptome changes. The early paraxial mesoderm differentiation stages can be efficiently recapitulated in vitro using mouse and human pluripotent stem cells. While Wnt activation alone can induce posterior PSM markers, acquisition of a committed PSM fate and efficient...

..., high-throughput assay to induce peri-gastrulation-associated patterning in geometrically confined human pluripotent stem cell (hPSC) colonies. We observed that, upon BMP4 treatment, phosphorylated SMAD1 (pSMAD1) activity in the colonies organized into a radial gradient. We developed a reaction...

... of pluripotent stem cells (PSCs) through stimulation of developmental signaling pathways can generate mature somatic cell types for basic laboratory studies or regenerative therapies. However, there has been significant uncertainty regarding a method to separately derive lung versus thyroid epithelial lineages...

...Ge Guo; Ferdinand von Meyenn; Maria Rostovskaya; James Clarke; Sabine Dietmann; Duncan Baker; Anna Sahakyan; Samuel Myers; Paul Bertone; Wolf Reik; Kathrin Plath; Austin Smith Much attention has focussed on the conversion of human pluripotent stem cells (PSCs) to a more naïve developmental status...

... basis of skeletal muscle diseases, but also provide a roadmap for recapitulating skeletal myogenesis in vitro from pluripotent stem cells (PSCs). PSCs have become an important tool for probing developmental questions, while differentiated cell types allow the development of novel therapeutic strategies...