...Giovanni Canu; Rosamaria Correra; Guillermo Diez-Pinel; Raphaël F. P. Castellan; Laura Denti; Alessandro Fantin; Christiana Ruhrberg ABSTRACT During embryonic development, muscle tissues, skin, and a subset of vascular endothelial cells arise from Pax3 -expressing embryonic progenitors defined...

... Ltd 2023 Summary: Abrogation of BMP signaling in early murine embryonic limb muscle precursors impaired their migration and proliferation, accelerated myogenic lineage progression and caused an aberrant proximodistal muscle pattern. PAX3 SMAD6 BMP signaling Hox Myogenic progenitor...

...Alicia Mayeuf-Louchart; Didier Montarras; Catherine Bodin; Tsutomu Kume; Stéphane D. Vincent; Margaret Buckingham Pax3 and Foxc2 have been shown genetically to mutually repress each other in the mouse somite. Perturbation of this balance in multipotent cells of the dermomyotome influences cell fate...

...), which is crucial for voluntary swallowing, arise from Pax3 -expressing somite cells. In vivo Kaede lineage tracing in zebrafish reveals the migratory route of cells from the anteriormost somites to OSM and SHM destinations. Expression of pax3b , a zebrafish duplicate of Pax3 , is restricted...

... Neural crest Wnt Enhancer Pax3 Zic3 A major theme in developmental biology is the need for cells to integrate multiple signals to make decisions about their fate. To achieve this, regulatory DNA must be structured so developmental control genes are expressed only when a cell is in the correct...

... role of this latter cell type in myoblast guidance. By creating a new Flk1/Pax3 double reporter mouse line, we have established the precise timetable for angioblast and myoblast delamination/migration from the somite to the limb bud. This timetable was conserved when mouse PSM was grafted...

... and the inhibition of preplacodal genes such as Six1 . In addition, we demonstrate that Gbx2 is upstream of the neural fold specifiers Pax3 and Msx1 . Our results place Gbx2 as the earliest factor in the NC genetic cascade being directly regulated by the inductive molecules, and support the notion...

... phenotype. Ectopic expression of FOXD3 represses MITF in cultured neural crest cells and in B16-F10 melanoma cells. We also show that FOXD3 does not bind directly to the MITF promoter, but instead interacts with the transcriptional activator PAX3 to prevent the binding of PAX3 to the MITF promoter...

... in this novel two-component myogenic system facilitated the diversification of vertebrates. Muscle Zebrafish Myosin Slow Fibre Fast Head Fin mrf4 ( myf6 ) myod myf5 Myogenin Hedgehog prdm1 pax3 meox1 hsp90 mef2d Motility is a key determinant of evolutionary success...

... anomalous great arteries owing to a failure to establish the initial complement of branchial arch arteries in the caudal pharyngeal region. Pbx1 deficiency also results in the failure of cardiac OFT septation. Pbx1 -null embryos lose a transient burst of Pax3 expression in premigratory cardiac neural crest...

... for mammary line and placode formation. The mammary line is displaced dorsally, and formation of placode 3 is impaired in Pax3 ILZ/ILZ mutants, which do not form ventral somitic buds. Mammary line formation is impaired and placode 3 is absent in Gli3 Xt-J/Xt-J and hypomorphic Fgf10 mutants, in which...

... Epithelial to mesenchymal transition Muscle Myod Myf5 Pax3 Pax7 Somite The embryonic myotome is the source of all epaxial and hypaxial skeletal muscles. The mechanisms underlying myotome development were recently reevaluated ( Brent and Tabin,2002 ; Buckingham,2001 ; Hollway...

... is predominantly in the hypaxial somite, adjacent to labelled smooth muscle cells in the aorta. Analysis of Pax3 GFP/+ embryos shows that these cells are Pax3 negative but GFP positive, with fluorescent cells in the intervening region between the aorta and the somite. This is consistent with the direct migration...

... function of Pax3 and Zic1 determines the neural crest fate in the amphibian ectoderm. Pax3 and Zic1 are expressed in an overlapping manner in the presumptive neural crest area of the Xenopus gastrula, even prior to the onset of the expression of the early bona fide neural crest marker genes Foxd3 and Slug...

.... At the limb bud level, Six1 and Six4 homeogenes control early steps of myogenic cell delamination and migration from the somite through the control of Pax3 gene expression. Impaired in their migratory pathway, cells of the somitic ventrolateral dermomyotome are rerouted, lose their identity and die...

... that maintain expression of PAX7,PAX3 and FREK. Given this dual fate, we asked whether single dermomyotome precursors generate both dermal and mitotic myoblast precursors, or alternatively, whether these cell types derive from distinct epithelial founders. Inovo clonal analysis revealed that single dermomyotome...

... the somites into the leg bud ( Chevallier et al.,1977 ; Jacob et al.,1979 ). We have detected single spindle shaped cells that express Pax3 but not MyoD during this initial phase of movement( Amthor et al., 1998 ). However,the situation is different as the cloacal anlage extends from the ventral muscle...

... the myogenic determination genes XMyf5 , XMyoD and Pax3 in a pattern reminiscent of amniote dermomyotome. Thus, Xenopus somites have characteristics in common with both fish and amniotes that shed light on the evolution of somite differentiation. We propose a model for the evolutionary adaptation of myogenesis...

...Rita C. Milewski; Neil C. Chi; Jun Li; Christopher Brown; Min Min Lu; Jonathan A. Epstein Pax3 is a transcription factor that is required by Pre-migratory neural crest cells give rise to the peripheral nervous system, melanocytes, some vascular smooth muscle, and numerous other derivatives...

... of the scapular blade and of rostral ribs. Such dysmorphogenesis is preceded by ectopic Pax3 expression in the hypaxial part of the dermomyotome,a region from which the blade of the scapula normally derives. A mutant CPZ,lacking a critical active site glutamate, fails to induce Pax3 expression and does not cause...