... analysis of the nascent cerebellum of transgenic Pax2-EGFP mice to address this issue. We found that cerebellar interneuronal precursors regularly show spontaneous postsynaptic currents, indicative of synaptic innervation, well before settling in the molecular layer. In keeping with the sensitivity...

..., ocular coloboma. Defective FGF signaling does not abrogate Shh or BMP signaling, nor does it affect axial patterning of the optic vesicle. Instead, FGF signaling regulates Mitf and Pax2 in coordinating the closure of the optic fissure and optic disc specification, which is necessary for the outgrowth...

...Matthew N. McCarroll; Zachary R. Lewis; Maya Deza Culbertson; Benjamin L. Martin; David Kimelman; Alex V. Nechiporuk Pax gene haploinsufficiency causes a variety of congenital defects. Renal-coloboma syndrome, resulting from mutations in Pax2 , is characterized by kidney hypoplasia, optic nerve...

... is essential for correct development of all forebrain derivatives, as mice and human with compromised six3 or shh function develop with a profound forebrain defect termed holoprosencephaly (HPE) ( Krauss,2007 ). Zic2 Six3 Hedgehog signaling Pax2 Prethalamus Optic stalk Retina Zebrafish ©...

... for chick Pax2 plasmid; and Thomas Brand for chick Tbx18 and Cfc1 plasmids. Our thanks extend to Ken Zaret and Hideyuki Yoshitomi for microarray data. We also thank Didier Stanier, John R. Timmer, Robert Garriock and Izumi Oda for valuable comments on the manuscript. This work was supported in part...

... A ), all of which express the paired-domain transcription factor Pax2. Although it appears that Lhx1 and Lhx5 do not control the initial specification of the neuronal cell types in which they are expressed, we have found a cell-autonomous requirement for either Lhx1 or Lhx5 to maintain the expression...

... path to reach the hindlimb level where it induces metanephros(adult kidney) formation, while the remaining duct gives rise to the male genital tract (epidydimis, vas deferens). The transcription factors Pax2 and Pax8 are essential for the initiation of pro- and mesonephros development. In a cDNA...

... in those cells responsible for Crystallin secretion in Drosophila , i.e. the cone cells. However, regulation of the DC5 enhancer is carried out not by Pax6 , but by Pax2 ( D-Pax2 ; shaven –FlyBase) in combination with the Sox2 homologue SoxN . Both proteins (D-PAX2 and SOXN) bind cooperatively to the DC5...

..., further demonstrating that the Drosophila Eya-Six regulatory cassette is evolutionarily conserved during mammalian inner ear development. We also analyzed several other otic markers and found that the expression of Pax2 and Pax8 was unaffected in Six1 -/- otic vesicle. By contrast, Six1 is required...

... of the mesenchyme. These results indicate that Six1 plays an essential role in early kidney development. In Six1 -/- kidney development, we have found that Pax2, Six2 and Sall1 expression was markedly reduced in the metanephric mesenchyme at E10.5,indicating that Six1 is required for the expression of these genes...

...Nicole Bäumer; Till Marquardt; Anastassia Stoykova; Derek Spieler; Dieter Treichel; Ruth Ashery-Padan; Peter Gruss The transcription factors Pax2 and Pax6 are co-expressed in the entire optic vesicle (OV) prior and concomitant with the establishment of distinct neuroretinal, retinal, pigmented...

... signaling modulates the size of the Pax2 + astrocyte precursor cell population at the optic disc in vitro. Together, these data provide a novel insight into the source of Hh that promotes neuroepithelial cell development in the mammalian optic disc and stalk. * Author for correspondence (e...

...-follicular group of cells. We show that pax2.1 and pax8, members of the zebrafish pax2/5/8 paralogue group, are expressed in the thyroid primordium. Whereas in mice, only Pax8 has a function during thyroid development, analysis of the zebrafish pax2.1 mutant no isthmus ( noi –/– ) demonstrates that pax2.1...

... suggest that spg / pou2 is a transcription factor that mediates regional competence to respond to Fgf8 signaling. *Author for correspondence (e-mail: [email protected] ) 27 11 2001 © 2002. 2002 Competence Fgf8 pou2 Oct3/4 Pou5f1 MHB Isthmus pax2 spiel-ohne-grenzen...

...Peter L. Pfeffer; Bernhard Payer; Gerlinde Reim; Marina Pasca di Magliano; Meinrad Busslinger Pax2 is the earliest known gene to be expressed throughout the mid-hindbrain region in late gastrula embryos of the mouse and is essential for the formation of an organizing center at the midbrain...

... tyrosine kinase RET and glial cell-derived neurotrophic factor (GDNF) form a signaling complex that is essential for ureteric bud growth and branching morphogenesis of the ureteric bud epithelium. We demonstrate that Pax2 expression in the metanephric mesenchyme is independent of induction by the ureteric...

.... and Busslinger, M. ( 1997 ). The characterization of novel Pax genes of the sea urchin and Drosophila reveal an ancient evolutionary origin of the Pax2/5/8 subfamily. Mech. Dev . 67 , 179 -192. Dai, X., Schonbaum, C., Degenstein, L., Bai, W., Mahowald, A. and Fuchs, E. ( 1998 ). The ovo gene...

... and nodose) form visceral sensory neurons that innervate taste buds and visceral organs. In the chick embryo, the ophthalmic trigeminal (opV) placode expresses the paired homeodomain transcription factor Pax3 from very early stages, while the epibranchial placodes express Pax2. Here, we show that Pax3...

...Eiji Matsunaga; Isato Araki; Harukazu Nakamura ABSTRACT Transcriptional factors and signaling molecules are responsible for regionalization of the central nervous system. In the early stage of neural development, Pax6 is expressed in the prosencephalon, while En1 and Pax2 are expressed...

..., the endogenous Ol-eng2, Pax2, Wnt1 and Fgf8 , which are essential components of the MHB genetic cascade, were ectopically expressed in this region. Therefore, we propose that Ol-eng2 induces de novo formation of an isthmus-like structure, which correlates with the development of ectopic midbrain structures...