... are developed and used to assess gene function in human pluripotent stem cells and their differentiated progeny. Human pluripotent stem cells shRNA CRISPR/Cas9 OCT4 POU5F1 T, brachyury DPY30 European Research Council 10.13039/501100000781 Cambridge University Hospitals...

... as a transcription factor mediating Nodal signaling via Smad2/3. Finally, we propose an evolutionarily conserved interaction between Foxh1 and PouV, a mechanism observed in Pou5f1-mediated regulation of pluripotency in human embryonic stem and epiblast cells. * Author for correspondence ( [email protected]...

...-mail: [email protected] ) 3 3 2006 © 2006. 2006 Gastrulation Self-renewal Embryonic stem cell Lineage commitment Pou protein Pou5f1 Xenopus Germ layer specification involves an elaboration of intrinsic cell-autonomous factors and non-autonomous signalling...

... and the trophectoderm (TE) that gives rise to the trophoblast lineage. Commitment to ICM lineage is attributed to the function of the two transcription factors, Oct4 (encoded by Pou5f1 ) and Nanog. However, a positive regulator of TE cell fate has not been described. The T-box protein eomesodermin (Eomes...

... comparison, and expression and functional data, we suggest that pou2 is the zebrafish ortholog of mouse Oct3/Oct4 and human POU5F1. For the mammalian genes, a function in brain development has so far not been described. In the absence of functional pou2 , expression of markers for the midbrain, MHB...

... Competence Fgf8 pou2 Oct3/4 Pou5f1 MHB Isthmus pax2 spiel-ohne-grenzen Hindbrain Zebrafish The organization of the vertebrate brain and its differentiation into functionally and anatomically distinct areas is based on early patterning and regional specification of the neural plate...