...-licence-1-1/ Summary: Expression of L1 transposons in germ cells of the human fetal testis is developmentally regulated and heterogeneous, and corresponds with upregulation of PIWI/piRNA repression machinery and accumulation of H3K9me3. LINE-1 piRNA PIWI Transposon Human fetal germ...

...Eva-Maria Weick; Eric A. Miska Distinguishing self from non-self plays a crucial role in safeguarding the germlines of metazoa from mobile DNA elements. Since their discovery less than a decade ago, Piwi-interacting RNAs (piRNAs) have been shown to repress transposable elements in the germline...

... restricted to the S phase of eukaryotic cells, is extended during the cell cycle of neoblasts. The planarian PIWI homologs SMEDWI-1 and SMEDWI-3 are required for proper localization of germinal histone H4 (gH4) mRNA to chromatoid bodies. The association between histone mRNA and chromatoid body components...

..., reduced animal size, reduced number of neoblasts, fewer chromatoid bodies and increased levels of transposon and repetitive-element transcripts. Our results suggest that PIWI family member SMEDWI-3 is one sDMA-containing chromatoid body protein for which methylation depends on PRMT5. Additionally, we...

...Andrea L. Zamparini; Marie Y. Davis; Colin D. Malone; Eric Vieira; Jiri Zavadil; Ravi Sachidanandam; Gregory J. Hannon; Ruth Lehmann In Drosophila , Piwi proteins associate with Piwi-interacting RNAs (piRNAs) and protect the germline genome by silencing mobile genetic elements. This defense system...

... and piwi . In vivo lineage mapping of the cells awaits genetic manipulation of the lineage, but previous research has demonstrated that the germline is not specified at the fourth division because animals are fertile even when micromeres, the parent blastomeres of small micromeres, are deleted. Here, we...

... other taxa segregate their germline after embryogenesis from multipotent progenitor cells. An overlapping set of genes, including vasa, nanos and piwi , operate in both multipotent precursors and in the germline. As we propose here, this conservation implies the existence of an underlying germline...

...Satomi Kuramochi-Miyagawa; Tohru Kimura; Takashi W. Ijiri; Taku Isobe; Noriko Asada; Yukiko Fujita; Masahito Ikawa; Naomi Iwai; Masaru Okabe; Wei Deng; Haifan Lin; Yoichi Matsuda; Toru Nakano The piwi family genes, which are defined by conserved PAZ and Piwi domains, play important roles in stem...

...Daniel N. Cox; Anna Chao; Haifan Lin ABSTRACT piwi represents the first class of genes known to be required for stem cell self-renewal in diverse organisms. In the Drosophila ovary, piwi is required in somatic signaling cells to maintain germline stem cells. Here we show that piwi encodes a novel...