... polybasic domain that electrostatically binds plasma membrane phosphoinositides, which is crucial for Dlg to regulate both polarity and tumorigenesis. Dlg Lgl Scrib aPKC Par-6 Polybasic domain Phosphoinositides PI4P PI(45)P 2 Cell polarity Tumorigenesis Drosophila To make ubi...

...Andrea Leibfried; Sandra Müller; Anne Ephrussi Polarity of the Drosophila oocyte is essential for correct development of the egg and future embryo. The Par proteins Par-6, aPKC and Bazooka are needed to maintain oocyte polarity and localize to specific domains early in oocyte development. To date...

... determinant PAR-6. Our results demonstrate that AP-1 controls an apical trafficking pathway required for the maintenance of epithelial polarity in vivo in a tubular epithelium. CDC-42 can interact with PAR-6 and recruit it to the anterior cortex of the one-cell C. elegans embryo ( Gotta et al., 2001...

... to remove PAR-3 from C. elegans embryos and examine its cellular role as intestinal precursor cells become polarized epithelial cells. At initial stages of polarization, PAR-3 accumulates in cortical foci that contain E-cadherin, other adherens junction proteins, and the polarity proteins PAR-6 and PKC-3...

... poles of the cell. Oocyte polarity has been proposed to depend on the same PAR proteins that generate AP polarity in C. elegans , with a complex of Bazooka (Baz; Par-3), Par-6 and aPKC marking the anterior and lateral cortex, and Par-1 defining the posterior. The function of the Baz complex in oocyte...

... together. The interacting proteins Par-6 (a PDZ and CRIB domain protein) and aPKC (an atypical protein kinase C) localize apically in fly and mammalian epithelial cells and are important for apicobasal polarity and junction formation. Caenorhabditis elegans PAR-6 and PKC-3/aPKC also localize apically...

... and maintaining epithelial cell polarity. However,less is known about what becomes of epithelial polarity proteins when various cell types become migratory and invasive. Here, we report the localization of several apical epithelial proteins, Par-6, Par-3/Bazooka and aPKC, during border cell migration...

..., such as PAR-3 and PAR-6, redistribute to the outer, apical surfaces of cells, whereas posteriorly localized PAR proteins, such as PAR-1 and PAR-2, redistribute to the inner, basolateral surfaces. Because PAR proteins are provided maternally, distinguishing apicobasal from earlier anterior-posterior functions...

... of rhabdomeres and AJs. We show that the Crb complex consisting of Crb, Stardust (Sdt) and Discs-lost (Dlt) colocalizes with another protein complex containing Par-6 and atypical protein kinase C (aPKC) in the rhabdomere stalk of photoreceptors. Loss of each component of the Crb complex causes age-dependent...

... Apical-basal polarity Gastrulation Ingression Morphogenesis Cell fate Cell shape Non-muscle myosin LIT-1 HMR-1 NMY-2 PAR-2 PAR-3 PAR-6 C. elegans Numerous studies on C. elegans embryos have revealed the basic strategies that establish the anterior-posterior and dorsal-ventral...

...Tak-June Hung; Kenneth J. Kemphues The par genes are required to establish polarity in the Caenorhabditis elegans embryo. Mutations in two of these genes, par-3 and par-6 , exhibit similar phenotypes. A third gene, pkc-3 , gives a similar phenotype when the protein is depleted by RNA interference...

... identity of early blastomeres. Maternal-effect lethal mutations with a partitioning defective phenotype ( par ) have identified several genes involved in this process. We have identified a new gene, par-6 , which acts in conjunction with other par genes to properly localize cytoplasmic components...