... . Wormbook . /10.1895/wormbook.1.170.1 Brangwynne , C. P. , Eckmann , C. R. , Courson , D. S. , Rybarska , A. , Hoege , C. , Gharakhani , J. , Julicher , F. and Hyman , A. A. ( 2009 ). Germline P granules are liquid droplets that localize by controlled dissolution...

...-3 function in parallel to restrict the kinase activity of the cell polarity regulator PAR-1 to the posterior cytoplasm of C. elegans zygotes. Polarity Kinase PAR proteins MEX-6 P granules PAR-3 National Institutes of Health 10.13039/100000002 R37HD037047 F32GM117814...

...Anne C. Campbell; Dustin L. Updike Germ granules (P granules) in C. elegans are required for fertility and function to maintain germ cell identity and pluripotency. Sterility in the absence of P granules is often accompanied by the misexpression of soma-specific proteins and the initiation...

... meiosis. Here we show that, despite their extensive sequence similarity, FBF-1 and FBF-2 have different effects on target mRNAs. FBF-1 promotes the degradation and/or transport of meiotic mRNAs out of the stem cell region, whereas FBF-2 prevents translation. FBF-2 activity depends on the P granule...

...Ujwal Sheth; Jason Pitt; Shannon Dennis; James R. Priess Germline-specific granules of unknown function are found in a wide variety of organisms, including C. elegans , where they are called P granules. P granules are cytoplasmic bodies in oocytes and early embryos. Throughout most of the C...

.... elegans eIF4E, IFE-1, is enriched in the germline and is a component of germ granules (P granules). The association of IFE-1 with P granules requires the P-granule protein PGL-1. In vitro PGL-1 interacts directly with IFE-1, but not with the other four isoforms of eIF4E. Analysis of animals depleted...

... and early embryo, and is localized to P granules and other possible mRNA-protein particles. cgh-1 is required for oocyte and sperm function. It is also needed to prevent the physiological germline apoptosis mechanism killing essentially all developing oocytes, making lack of cgh-1 function the first...

...Jennifer A. Schisa; Jason N. Pitt; James R.Priess ABSTRACT P granules are cytoplasmic structures of unknown function that are associated with germ nuclei in the C. elegans gonad, and are localized exclusively to germ cells, or germ cell precursors, throughout the life cycle. All the known protein...

... in four-to 24-cell embryos, where it is localized in a crescent at the junction between the germline cell and its neighboring gut cell. This is the region of P 2 and P 3 to which the spindle and P granules must move to ensure normal division asymmetry and cytoplasmic partitioning. Indeed, during early...

... from a maternal RNA associated with P granules, and is required for the efficient incorporation of PGCs into the somatic gonad. nos-1 is expressed in PGCs after gastrulation, and is required redundantly with nos-2 to prevent PGCs from dividing in starved animals and to maintain germ cell viability...

... PIE-1 MES-2 MES-6 transcriptional repression P granules germline Caenorhabditis elegans The mechanisms that set the germline apart from the soma have fascinated biologists since the work of Weismann in the late 1800s ( Weismann, 1893 ). Several developmental characteristics distinguish...

...Susana Guedes; James R. Priess ABSTRACT In the nematode Caenorhabditis elegans , germ cells arise from early embryonic cells called germline blastomeres. Cytoplasmic structures called P granules are present in the fertilized egg and are segregated into each of the germline blastomeres during...

...Steven N. Hird; Janet E. Paulsen; Susan Strome ABSTRACT Germ granules are ribonucleoprotein particles that are thought to function in germline specification in invertebrates and possibly in vertebrates. In Caenorhabditis elegans , these structures, termed P granules, are partitioned to the germline...

... with the germlinespecific P granules. We observed embryonically transcribed RNAs in somatic cells as early as the 4-cell stage. These transcripts were not detected in germline cells. These observations suggest that mechanisms which distinguish between soma and germline cause asymmetries in mRNA stability and transcription...