... that these enhancers indeed regulate Otx2 expression in forebrain and midbrain. Development of mesencephalic and diencephalic regions was differentially regulated in a dose-dependent manner by the cooperation between Otx1 and Otx2 under FM and FM2 enhancers: the more caudal the structure the higher the OTX dose...

... 1-mediated neural fate determination and is required for induction or proper patterning of gene expression related to sensory organ morphogenesis ( Otx1 and Bmp4 , respectively). Tbx1 loss-of-function causes dysregulation of neural competence in otocyst regions linked to the formation of either...

... body and may also play a role in regulation of neuronal differentiation in the developing eye. Fig. 7. Downregulation of Otx1 expression. In wild-type mice, Otx1 transcripts were detected by in situ hybridization in the developing ciliary and iris epithelia at E18 (A), P1 (B) and P7 (C...

... anlage. Previous data suggest that both Otx1 and Otx2 might contribute to the establishment of the different eye territories. Here, we have analysed the ocular phenotype of mice carrying different functional copies of Otx1 and Otx2 and we show that these genes are required in a dose-dependent manner...

... from E15.5 and E18.5 mouse cerebral cortex. A novel cDNA, Svet1 , was cloned which was specifically expressed in the proliferating cells of the SVZ but not the VZ. The VZ is marked by the expression of the Otx1 gene. Later in development, Svet1 and Otx1 were expressed in subsets of cells of upper (II...

... in a reduction in the number of Otx1-positive neurons in this layer during postnatal development. Homozygous mice also show a reduction in the number of GAD67-positive interneurons throughout the cortex. Our results suggest that Querkopf is an essential component of a genetic cascade regulating cell...

...Hakim Morsli; Francesca Tuorto; Daniel Choo; Maria Pia Postiglione; Antonio Simeone; Doris K. Wu ABSTRACT The Otx1 and Otx2 genes are two murine orthologues of the Orthodenticle ( Otd ) gene in Drosophila . In the developing mouse embryo, both Otx genes are expressed in the rostral head region...

...Dario Acampora; Virginia Avantaggiato; Francesca Tuorto; Paolo Barone; Marzia Perera; Daniel Choo; Doris Wu; Giorgio Corte; Antonio Simeone Otx1 and Otx2 , two murine homologs of the Drosophila orthodenticle (otd) gene, show a limited amino acid sequence divergence. Their embryonic expression...

...Yoko Suda; Jun Nakabayashi; Isao Matsuo; Shinichi Aizawa Mice have two Otx genes, Otx1 and Otx2 . Prior to gastrulation, Otx2 is expressed in the epiblast and visceral endoderm. As the primitive streak forms, Otx2 expression is restricted to the anterior parts of all three germ layers. Otx1...

...Dario Acampora; Virginia Avantaggiato; Francesca Tuorto; Paola Briata; Giorgio Corte; Antonio Simeone ABSTRACT Otx1 and Otx2 , two murine homologs of the Drosophila orthodenticle (otd) gene, contribute to brain morphogenesis. In particular Otx1 null mice are viable and show spontaneous epileptic...

... and mammals. In Drosophila , mutational inactivation of the orthodenticle gene results in deletions in anterior parts of the embryonic brain and in defects in the ventral nerve cord. In the mouse, targeted elimination of Overexpression of either orthodenticle or the human OTX the homologous Otx2 or Otx1 genes...

... to the homeodomain suggesting that protein divergence outside the homeodomain might account for functional differences acquired during brain evolution. In order to gain insight into this possibility, we replaced the murine Otx1 gene with a Drosophila otd cDNA. Strikingly, epilepsy and corticogenesis defects due...

... Otx1 Otx2 At the beginning of the century, pioneering embryological experiments performed on amphibians by Spemann and collaborators showed that transplantation of a gastrula dorsal blastopore lip to a region fated to form ventral mesoderm, resulted in the formation of a secondary axis...