... to injury. Here, we monitored osteoblast-immune cell interactions in bone, a tissue which is particularly difficult to in vivo image in tetrapod species. Ablation of individual osteoblasts leads to recruitment of neutrophils and macrophages in varying numbers, depending on the extent of the initial insult...

... skeletal progenitor cells were particularly sensitive to a ciliopathic insult, undergoing unchecked passage through the cell cycle and subsequent increased proliferation. Furthermore, whereas neural crest-derived skeletal differentiation was initiated, osteoblast maturation failed to progress to completion...

... and DCHS1 result in Van Maldergem syndrome, which is characterised, in part, by craniofacial abnormalities. Here, we analyse the role of Dchs1-Fat4 signalling during osteoblast differentiation in mouse. We show that Fat4 and Dchs1 mutants mimic the craniofacial phenotype of the human syndrome and that Dchs1...

...Valerie S. Salazar; Satoshi Ohte; Luciane P. Capelo; Laura Gamer; Vicki Rosen Enhanced BMP or canonical Wnt (cWnt) signaling are therapeutic strategies employed to enhance bone formation and fracture repair, but the mechanisms each pathway utilizes to specify cell fate of bone-forming osteoblasts...

... developmental and regenerative bone formation and providing niche microenvironments for various cell types. Bone Angiogenesis Blood vessels Endothelial cell Chondrocyte Osteoblast Osteoclast Max-Planck-Gesellschaft 10.13039/501100004189 Westfälische Wilhelms-Universität Münster...

... bone regeneration in the lower jaw of adult zebrafish, we show that chondrocytes are crucial for generating thick bone during repair. During jawbone regeneration, we find that chondrocytes co-express genes associated with osteoblast differentiation and produce extensive mineralization, which...

... for correspondence ( [email protected] ) Competing interests The authors declare no competing or financial interests. 8 10 2015 18 3 2016 © 2016. Published by The Company of Biologists Ltd 2016 Summary: Conditional ablation of Fgfr1 and Fgfr2 in the osteoblast lineage in mouse...

... expand and are resorbed by osteoclasts then replaced by osteoblastic bone formation. Ephrin B2 is a receptor tyrosine kinase expressed by osteoblasts and growth plate chondrocytes that promotes osteoblast differentiation and inhibits osteoclast formation. We investigated the role of ephrin B2...

... the receptor Bmpr1a in osteoblast lineage cells with Dmp1-Cre, we observed a dramatic increase in trabecular bone mass in postnatal mice, which was due to a marked increase in osteoblast number that was likely to be driven by hyperproliferation of Sp7 + preosteoblasts. Similarly, inducible deletion of Bmpr1a...

...Takeshi Takarada; Ryota Nakazato; Azusa Tsuchikane; Koichi Fujikawa; Takashi Iezaki; Yukio Yoneda; Eiichi Hinoi Runt-related transcription factor 2 (Runx2) is an essential transcriptional regulator of osteoblast differentiation and its haploinsufficiency leads to cleidocranial dysplasia because...

... cell lineage-specific programs are protected from regenerative crosstalk between neighboring fin tissues. During fin regeneration, osteoblasts revert from a non-cycling, mature state to a cycling, preosteoblastic state to establish a pool of progenitors within the blastema. After several rounds...

... the existing bone, has remained unresolved. Here, we demonstrate that a retinoic acid (RA)-degrading niche is established by Cyp26a1 in the proximal basal epidermal layer that orchestrates ray-interray organization by spatially restricting osteoblasts. Disruption of this niche causes preosteoblasts to ignore...

.... Osteochondroprogenitor Osteoblast Skeletal stem cell Bone formation during embryonic development and postnatal life is a tightly regulated biological process ( Olsen et al., 2000 ). During embryonic development cells of the mesenchymal lineage migrate to specific anatomical locations and either directly...

...Karina Geurtzen; Franziska Knopf; Daniel Wehner; Leonie F. A. Huitema; Stefan Schulte-Merker; Gilbert Weidinger Zebrafish have an unlimited capacity to regenerate bone after fin amputation. In this process, mature osteoblasts dedifferentiate to osteogenic precursor cells and thus represent...

... intramembranous ossification in the skull. * Author for correspondence ( [email protected] ) Competing interests statement The authors declare no competing financial interests. 3 9 2012 © 2012. Published by The Company of Biologists Ltd 2012 Osteoblast Cranial bone Skull Wnt β...

...Nan Li; Robert N. Kelsh; Peter Croucher; Henry H. Roehl Although the regulation of osteoblast and adipocyte differentiation from mesenchymal stem cells has been studied for some time, very little is known about what regulates their appearance in discrete regions of the embryo. Here we show...

...Christina Lindsey Hammond; Stefan Schulte-Merker Hedgehog (Hh) signalling has been implicated in the development of osteoblasts and osteoclasts whose balanced activities are critical for proper bone formation. As many mouse mutants in the Hh pathway are embryonic lethal, questions on the exact...

... is largely unknown. Here, we report that Hand2 negatively regulates intramembranous ossification of the mandible by directly inhibiting the transcription factor Runx2, a master regulator of osteoblast differentiation. Hand proteins physically interact with Runx2, suppressing its DNA binding...

... is completely phenocopied by treating 4 dpf wild-type embryos with either RA or the pharmacological Cyp26 blocker R115866, thus identifying a previously unappreciated role for RA and cyp26b1 in osteogenesis of the vertebral column. Cyp26b1 is expressed within osteoblast cells,demonstrating that RA levels within...

...Kathrin Laue; Martina Jänicke; Nikki Plaster; Carmen Sonntag; Matthias Hammerschmidt Skeletal syndromes are among the most common birth defects. Vertebrate skeletogenesis involves two major cell types: cartilage-forming chondrocytes and bone-forming osteoblasts. In vitro, both are under...