... as stably replatable cystic spheroids in addition to differentiated organoids. Contrary to classical EDTA-derived organoids, these spheroids display poor intestinal differentiation and are independent of Rspondin/Noggin/EGF for growth. Their transcriptome resembles strikingly that of fetal intestinal...

...) function when active ISCs (aISCs) are lost to damage. Here, we modeled fISC activation in mouse intestinal organoids with doxorubicin (DXR) treatment, a chemotherapeutic known to ablate Lgr5 + aISCs in vivo . Similar fISC gene activation was observed between organoids treated with low versus high DXR...

...Tania Hübscher; L. Francisco Lorenzo-Martín; Thomas Barthlott; Lucie Tillard; Jakob J. Langer; Paul Rouse; C. Clare Blackburn; Georg Holländer; Matthias P. Lutolf ABSTRACT Although the advent of organoids has opened unprecedented perspectives for basic and translational research, immune system...

... took place in Vienna in March 2024, covering topics such as early embryogenesis, nervous system development and disease, regeneration and the latest technologies. Early embryogenesis Organoids Single cell technologies Stem cells Deutsche Forschungsgemeinschaft http://dx.doi.org...

... in development of the proximal intestine. To identify how mutations in RFX6 impact intestinal patterning and function, we derived induced pluripotent stem cells from this patient to generate human intestinal organoids (HIOs). We identified that the duodenal HIOs and human tissues had mixed regional identity...

...Hahyung Y. Kim; Ishani Sinha; Karen E. Sears; Charlotte Kuperwasser; Gat Rauner ABSTRACT The varying pathways of mammary gland development across species and evolutionary history are underexplored, largely due to a lack of model systems. Recent progress in organoid technology holds the promise...

... restriction. However, regulatory mechanisms controlling development of placental epithelial cells, the trophoblasts, remain poorly elucidated. Using trophoblast stem cells (TSCs), trophoblast organoids (TB-ORGs) and primary cytotrophoblasts (CTBs) of early pregnancy, we herein show that autocrine NOTCH3...

... cells and neurons. Despite previously characterizing some cell types, many remain undefined. This study aimed to chart the in vitro development timeline of the inner ear organoid to understand the mechanisms at play. Using single-cell RNA sequencing at ten stages during the first 36 days...

... (DHFR), a key enzyme in 1C metabolism, is highly expressed in human and mouse neural progenitors at the early stages of neocortical development. Here, we have investigated the role of DHFR in the developing neocortex and report that reducing its activity in human neural organoids and mouse embryonic...

... development and disease. Previously, our group developed methods to produce prostate organoids using induced pluripotent stem cells (iPSCs). Here, we show that human iPSCs can be differentiated into prostate organoids using neonatal rat seminal vesicle mesenchyme in vitro . The organoids can be used to study...

... cell-derived organoids has provided insights into the rearrangements that eye progenitors undergo to adopt such a shape. Molecular and pharmacological interference with these rearrangements has further identified the underlying molecular machineries and the physical forces involved...

...Sabina Kanton; Sergiu P. Paşca ABSTRACT Deconstructing and then reconstructing developmental processes ex vivo is crucial to understanding how organs assemble and how physiology can be disrupted in disease. Human 3D stem cell-derived systems, such as organoids, have facilitated this pursuit...

...Anne Grapin-Botton; Yung Hae Kim ABSTRACT Organoids have become one of the fastest progressing and applied models in biological and medical research, and various organoids have now been developed for most of the organs of the body. Here, we review the methods developed to generate pancreas...

... human brain development and disease, pluripotent stem cell-derived models, such as organoids, provide tractable access to neurodevelopmental processes. Despite many strengths of neural organoid models, the extent of their replication of endogenous metabolic programs is currently unclear and requires...

... difficult to generate efficiently and reproducibly from mouse embryonic stem cells: definitive endoderm and neural organoids. We present new protocols for rapid generation of nearly pure definitive endoderm and forebrain-patterned neural organoids that model the development of prethalamic and hippocampal...

... clearance. These functions are executed deep within tissues, which are often poorly accessible and subject to environmental variability, especially in humans. Here, we discuss how multicellular 3D systems in a dish – organoids – are transitioning from a proof-of-principle approach to a timely, robust...

... reprogrammed cells in a degranulation-dependent manner. Importantly, in vivo partial reprogramming is strongly reduced by adoptive transfer of NK cells, whereas it is significantly increased by their depletion. Notably, in the absence of NK cells, the pancreatic organoids derived from OSKM-expressing mice...

... organoid systems can now provide unprecedented insights into the physical principles of human development. * Authors for correspondence ( [email protected] ; [email protected] ) Competing interests K.R.K. is an inventor on several patents and patent applications...

... and differentiation. Multiple distinct trophoblast states were identified, representing progenitor CTB, column CTB, SCT precursors and EVT. Lineage trajectory analysis identified a progenitor origin that was reproduced in human trophoblast stem cell organoids. Heightened expression of basal cell adhesion molecule...

..., resulting in complex morphogenesis in organoids ( Fig. 1 A,B) ( Brassard and Lutolf, 2019 ; Eiraku et al., 2011 ; Kleinman and Martin, 2005 ; Meinhardt et al., 2014 ). In gastruloids, precisely timed addition of 5% Matrigel to the culture medium induces embryo-like architecture with somite-like...