... its DNA-binding sites and transiently converts Tcf7/Lymphoid enhancer binding factor 1 (Lef1) into a transcriptional activator. One of the earliest and evolutionarily conserved functions of Wnt/β-Catenin signaling is the induction of the blastopore lip organizer. Here, we demonstrate...

...Alfonso Martinez Arias; Ben Steventon ABSTRACT Organizers, which comprise groups of cells with the ability to instruct adjacent cells into specific states, represent a key principle in developmental biology. The concept was first introduced by Spemann and Mangold, who showed...

... of FGF signals originated from the so-called isthmic organizer at the mid/hindbrain junction; however, the underlying mechanism is unclear. We show that deleting Ptpn11, which links FGF with the ERK pathway, prevents inferior colliculus formation by depleting a previously uncharacterized stem cell zone...

... of transcription initiation that involves mesoderm and organizer gene expression. Using TBP family single- and triple-knockdown experiments, α-amanitin treatment, transcriptome profiling and chromatin immunoprecipitation, we found that TFI gene expression cannot be explained by functional redundancy, is supported...

... statement The authors declare no competing financial interests. 29 6 2010 © 2010. 2010 Wnt Tcf β-catenin Axis formation Posterior patterning Hydractinia Cnidaria RNAi Metamorphosis Regeneration Organizer Invertebrate Oocytes Gonads Sperm maturation Sperm development...

...Caroline Vachias; Jean-Louis Couderc; Muriel Grammont Organisers control the patterning and growth of many tissues and organs. Correctly regulating the size of these organisers is crucial for proper differentiation to occur. Organiser activity in the epithelium of the Drosophila ovarian follicle...

...Yu-Huan Shih; Cheng-Liang Kuo; Caroline S. Hirst; Chris T. Dee; Yu-Ru Liu; Zulfiqar Ali Laghari; Paul J. Scotting Formation of the organizer is one of the most central patterning events in vertebrate development. Organizer-derived signals are responsible for establishing the CNS and patterning...

... addressed these issues in Xenopus embryos by manipulating the timing and location of dorsal organizer induction: the primary dorsal organizer was ablated by UV irradiation, and a new organizer was induced at various locations, either early, by mechanical rotation, or late, by injection of lithium chloride...

...Jean-Daniel Fauny; Bernard Thisse; Christine Thisse Formation of the vertebrate embryo is known to depend on the activity of organizing centers. The dorsal Spemann organizer is the source of growth factor antagonists that participate in the creation of signaling gradients. In various species...

...Monica Dixon Fox; Ashley E. E. Bruce The organizer is essential for dorsal-ventral (DV) patterning in vertebrates. Goosecoid (Gsc), a transcriptional repressor found in the organizer, elicits partial secondary axes when expressed ventrally in Xenopus, similar to an organizer transplant. Although...

...Pablo Oteíza; Mathias Köppen; Miguel L. Concha; Carl-Philipp Heisenberg Handedness of the vertebrate body plan critically depends on transient embryonic structures/organs that generate cilia-dependent leftward fluid flow within constrained extracellular environments. Although the function...

... of the zebrafish organizer. We show that FGFs function downstream of squint and bozozok to turn on chordin expression. Full rescue of ichabod by Squint is dependent on FGF signaling, and partial rescue by FGFs is completely dependent on chordin . By contrast, Bozozok can rescue the complete anteroposterior axis...

... in the regionalization of the ectoderm, they are thought to act during gastrulation to control, in the mesendoderm, the expression of organizer signals. We discuss the evolution of chordate ectodermal patterning in light of our findings. Therefore, the ability to express Ci-sFRP1/5 at the 64-cell stage appears...

..., and administration of MO2 to ichabod embryos increases the extent of ventralization. MOs directed against β- catenin-1 (MO1), by contrast, had no ventralizing effect on wild-type embryos. β- catenin-2 is thus specifically required for organizer formation and this function is apparently required maternally, because...

... during normal neural plate formation as it limits the number of chordin - and Xshh -expressing cells in the prospective notochord and floorplate,which act as organizing centers. Our findings show that Barhl2 is part of a pathway regulating EPCD. They also provide evidence that apoptosis plays...

... determinants VegT Xwnt8 Organizer Cytoplasmic determinants, which are located in particular regions of the egg, drive the cell fate during development. Although the non-cell autonomous process acting through cell-cell interaction has been emphasized in the early development of chordates ( Gilbert...

... Sonic hedgehog (Shh), a secreted protein, and requires direct cellular contact between the notochord and the neural tube. Another model proposes a role for the organizer in generating midline precursor cells that produce floor plate cells independent of notochord specification, and proposes that floor...

.... Additionally, we observed ectopic expression of Wnt1 in the embryonic roofplate organizer region and dorsal telencephalon. To determine the phenotypic consequences of such Wnt1 misexpression, we deleted a putative EMX2 DNA-binding site from the Wnt1 enhancer and used this to misexpress Wnt1 in the developing...

... parts of the organizer region were explanted at gastrula stage and tested for their inducing/patterning activities on either naive ectoderm or on midgastrula stage dorsal ectoderm. We show that the anterior dorsal mesendoderm of the organizer region has a weak neural inducing activity compared...

... for correspondence (e-mail: [email protected] ) 29 5 2002 © 2002. 2002 XTcf3 Antisense oligo Xenopus Organizer VegT The T cell factor (TCF) family of DNA binding proteins plays key roles in both embryonic development and in tumor progression. TCFs act as effectors of Wingless/Wnt...