... attributed. Summary: Optogenetic tools can be used to determine how transcription factor levels and nuclear retention impact a dynamic patterning process. Dorsal transcription factor Drosophila melanogaster LEXY and B-LID Dorsal-ventral patterning Gene expression dynamics Optogenetics...

... cell states. By simultaneously imaging the physiological dynamics of transcription and signalling, we show the jump coincides with the onset of collective oscillations of cAMP. Optogenetic control of cAMP pulses shows that different jump genes respond to distinct dynamic features of signalling. Late...

...Emily K. Ho; Harrison R. Oatman; Sarah E. McFann; Liu Yang; Heath E. Johnson; Stanislav Y. Shvartsman; Jared E. Toettcher ABSTRACT Positional information in development often manifests as stripes of gene expression, but how stripes form remains incompletely understood. Here, we use optogenetics...

.... However, mechanistic insight into this dynamic self-organization is limited by a lack of methods for spatiotemporal control of signaling, and the relevance of signal dynamics and cell-to-cell variability to pattern emergence remains unknown. Here, we combine optogenetic stimulation, imaging...

... an optogenetic approach to control NICD release (OptIC-Notch) and monitored the subsequent complex formation and target gene activation. Strikingly, we observed that, when uncleaved, OptIC-Notch sequestered CSL in the cytoplasm. Hypothesising that exposure of a juxta membrane ΦWΦP motif is key to sequestration...

... as a proxy for the kinase activity. Single fluorophore-based biosensors, such as KTRs, enable multiplexed imaging of kinase activity at the single-cell level ( Regot et al., 2014 ; Maryu et al., 2016 ; Jacques et al., 2021 ) and combination with optogenetics ( Zhou et al., 2017 ; Goglia et al., 2020...

... generation at apical cell sides, but several studies indicate a role of mechanical tension at lateral cell sides in this process. However, whether lateral tension increase is sufficient to drive epithelial folding remains unclear. Here, we have used optogenetics to locally increase mechanical force...

...- regulatory elements and the conditionality of optogenetics. Our studies also demonstrate differences between the nitroreductase and M2 systems that influence their efficacies for specific applications. Using this integrative approach, we have ablated cells in zebrafish embryos with both spatial and temporal...

... in optogenetics are allowing protein function to be controlled with the precision of a pulse of laser light in vivo , providing a powerful new tool to perturb developmental processes at a wide range of spatiotemporal scales. In this Primer, we describe the most commonly used optogenetic tools, their application...

...Anna Reade; Laura B. Motta-Mena; Kevin H. Gardner; Didier Y. Stainier; Orion D. Weiner; Stephanie Woo ABSTRACT Here, we describe an optogenetic gene expression system optimized for use in zebrafish. This system overcomes the limitations of current inducible expression systems by enabling robust...

... and differentiation. The lack of tools to manipulate signaling pathways dynamically in space and time has been a major technical challenge for biologists. Optogenetic techniques, which utilize light to control protein functions in a reversible fashion, hold promise for modulating intracellular signaling networks...

... than 24 h. Accumulating evidence, together with recently developed optogenetic approaches, suggests that such ultradian oscillators play important roles during cell fate decisions, and analyzing the functional links between ultradian oscillation and cell fate determination will contribute to a deeper...