... of the pronucleus. Although many factors are involved in the regulation of oocyte maturation, this process is principally governed by maturation-promoting factor (MPF) ( Jones, 2004 ). MPF is a complex of cyclin B1 and Cdk1 that orchestrates progression through both mitosis and meiosis ( Morgan, 1995 ; Nurse...

...Jiawei Sun; Lu Yan; Weimin Shen; Anming Meng ABSTRACT Maternal mRNAs and proteins dictate early embryonic development before zygotic genome activation. In the absence of transcription, elaborate control of maternal mRNA translation is of particular importance for oocyte maturation and early...

... conserved between species. By contrast, the few known maturation-inducing hormones (MIHs) that act on oocytes to initiate this process are highly variable in their molecular nature. Using the hydrozoan jellyfish species Clytia and Cladonema , which undergo oocyte maturation in response to dark-light...

... the axis in terms of cellular components. This polarization occurs during oocyte maturation. Oocytes within the gonad have the germinal vesicle (GV) close to the future animal pole. When the GVs of full-grown oocytes were experimentally translocated to the opposite pole by centrifugal force, every aspect...

...Qian-Qian Sha; Xing-Xing Dai; Yujiao Dang; Fuchou Tang; Junping Liu; Yin-Li Zhang; Heng-Yu Fan ABSTRACT Mammalian oocyte maturation depends on the translational activation of stored maternal mRNAs upon meiotic resumption. Cytoplasmic polyadenylation element binding protein 1 (CPEB1) is a key oocyte...

... processed and T EPAC VV expression was assessed by western blot using anti-GFP antibody. (B) I CNG-CaCC measured in CNG-expressing oocytes in the presence (four oocytes) or absence of T EPAC VV (five oocytes), before or after injection of 0.8 pmol db-cAMP. (C) Levels of oocyte maturation...

.... The anti-UPIII extracellular domain antibody binds more effectively to the surface of mature than immature ovarian oocytes. We propose that the structural and functional competency of the UPIII-Src signaling system in MDs is strictly regulated during oocyte maturation and subsequently in sperm-mediated egg...

...Wassim El-Jouni; Shirley Haun; Rawad Hodeify; Azida Hosein Walker; Khaled Machaca Vertebrate oocytes are maintained in meiotic arrest for prolonged periods of time before undergoing oocyte maturation in preparation for fertilization. Cyclic AMP (cAMP) signaling plays a crucial role in maintaining...

...Anna E. Burrows; Bonnielin K. Sceurman; Mary E. Kosinski; Christopher T. Richie; Penny L. Sadler; Jill M. Schumacher; Andy Golden Maturation promoting factor (MPF), a complex of cyclin-dependent kinase 1 and cyclin B, drives oocyte maturation in all animals. Mechanisms to block MPF activation...

... signal, the major sperm protein (MSP), to induce oocyte meiotic maturation and ovulation. MSP binds to multiple receptors, including the VAB-1 Eph receptor protein-tyrosine kinase on oocyte and ovarian sheath cell surfaces. Canonical VAB-1 ligands called ephrins negatively regulate oocyte maturation...

... Endocytosis cAMP Oocyte maturation Sea urchin Eggs of most animal species are fertilized by a single sperm and fusion of these two cells is essential for the activation of the developmental program of the zygote. Cases in which multiple sperm fertilize an egg, a condition known as polyspermy...

... is involved, not only in the MAPK cascade pathway, but also in a mechanism that directly activates MPF in Xenopus oocytes. MAPK Mos MPF Myt1 Oocyte maturation Xenopus In animal kingdom, oocytes arrest the cell cycle at the G2 prophase boundary of the first meiotic cycle. Then, they grow...

... of c-mos mRNA is necessary for oocyte maturation in the mouse. EMBO J . 13 , 5712 -5720. Giet, R. and Prigent, C. ( 1999 ). Aurora/Ipl1p-related kinases, a new oncogenic family of mitotic serine-threonine kinases. J. Cell Sci . 112 , 3591 -3601. Gingras, A. C., Raught, B...

... in the context of a universal mechanism for meiotic maturation in oocytes throughout the animal kingdom. * Author for correspondence (e-mail: [email protected]) 05 08 1999 27 09 1999 © 1999 by Company of Biologists 1999 MAP kinase MPF Oocyte maturation Xenopus...

... to progesterone, that GSK-3β can specifically block progesterone-mediated oocyte maturation, and that GSK-3β inhibition is a rate-limiting step in progesterone-mediated maturation. Xdsh in pSP64R1, human GSK-3β WT and KM in pSP64T and the expression vectors for Xenopus β-catenin and Δβ-catenin, pXBC40...

... membrane ( Angres et al., 1991 . Development 111, 829-844). To assess the origin of this unexpected distribution in the egg, the accumulation and localization of the cadherin during oogenesis and oocyte maturation were investigated. We report here that U-cadherin is present in Xenopus oocytes throughout...

... laevis oligodeoxyribonucleotides oocyte maturation protein synthesis maternal mRNA D7 A major focus of modern cell and developmental biology is the characterization of protein function. With respect to this goal, some of the most promising new approaches have been provided...

...L. Dennis Smith 21 09 1989 © 1989 by Company of Biologists 1989 oocyte maturation induction transmembrane signaling regulation cell cycle MPF amphibia The induction of oocyte maturation is known to involve an initial action of agonists at the oocyte surface...

... chorionic gonadotropin at the same time. After oocyte maturation, donor oocytes were stained with 0·00125 % Nile blue or 0·0025 % neutral red for 15 min and transferred surgically to host females anesthetized with MS222. Our usual schedule was progesterone treatment of donor oocytes at 10p.m. at 18°C, hCG...

...Jean Gautier; Renée Tencer ABSTRACT Patterns of protein phosphorylation and synthesis during axolotl ( Ambystoma mexicanum ) oocyte maturation were studied by incorporation of [ 32 P]orthophosphate and [ 35 S]methionine into polypeptides, followed by two-dimensional gel electrophoresis. Various...