... that these progenitors coordinately establish primitive hematopoiesis. Using Oct3/4 promoter-driven GFP transgenic mice,early endothelial progenitors, stroma-dependent hematopoietic progenitors, and CFU-c were all shown to express the Oct3/4 transcription factor. Among Oct3/4-positive cells, both endothelial...

...Koji Shimozaki; Kinichi Nakashima; Hitoshi Niwa; Tetsuya Taga Oct3/4 plays a critical role in maintaining embryonic stem cell pluripotency. Regulatable transgene-mediated sustained Oct3/4 expression in ES cells cultured in serum-free LIF-deficient medium caused accelerated differentiation...

..., and expression of pou2 mRNA itself is reduced in spg -homozygous embryos at this stage. These experiments suggest two roles for pou2 . Initially, Pou2 functions during early proliferation and morphogenesis of the blastomeres, similar to Oct3/4 in mammals during formation of the inner cell mass. During zebrafish...

... in situ hybridization) were documented on a Zeiss axiophot. cDNA of murine Oct3/4, fgf8, pou2 and nuclear lacZ , subcloned into pCS2+ ( Rupp et al., 1994 ), were linearized and transcribed using the SP6 message mMachine kit (Ambion). The amount of mRNA injected was estimated from...