Fig. 1. The larvacean Oikopleura dioica is a fast-developing chordate. (A) Developmental characteristics. In embryogenesis, each cell is restricted to give rise to a single and invariant tissue cell fate at the 32- and 110-cell stages in O. dioica and the ascidian Halocynthia roretzi , resp... More about this image found in The larvacean Oikopleura dioica is a fast-developing chordate. (A) Devel...

Fig. 2. Gene expression profiles during Oikopleura dioica and Halocynthia roretzi development. (A-C) Clustering of gene expression patterns. Using the staged RNA-seq data, the changes in 13,924 ( O. dioica ) and 12,499 ( H. roretzi ) transcripts were patterned into 12 groups using k-means cl... More about this image found in Gene expression profiles during Oikopleura dioica and Halocynthia roretz...

Fig. 3. Clustering of transcription factor expression profiles during Oikopleura dioica and Halocynthia roretzi development. Changes in the 381 ( O. dioica ) and 444 ( H. roretzi ) transcription factor (TF) mRNAs were classified into nine groups using k-means clustering and classified into s... More about this image found in Clustering of transcription factor expression profiles during Oikopleura d...

Fig. 4. Zygotic expression of transcription factor genes from the 32-cell embryos to 1.5 hpf embryos in Oikopleura dioica . Whole-mount in situ hybridization detected the expression of 25 out of 81 transcription factor (TF) genes in the 32-cell embryos. Arrowheads indicate the presumptive not... More about this image found in Zygotic expression of transcription factor genes from the 32-cell embryos t...

Fig. 5. Isolation of vegetally enriched maternal transcripts. (A) The 8-cell embryos were dissected into animal and vegetal hemispheres. The RNA-seq samples were taken immediately after dissection (B) or at periods corresponding to late larvae stage ( Oikopleura dioica ) or the 110-cell stage ( ... More about this image found in Isolation of vegetally enriched maternal transcripts. (A) The 8-cell embry...

Fig. 6. Oikopleura dioica has postplasmic RNAs . (A-E) Whole-mount in situ hybridization (WISH) of 8-cell embryos. Five vegetally enriched maternal transcripts were detected in vegetal and posterior blastomeres (blue arrowheads). (F-I″) Fluorescent WISH. Orientations of 8-cell embryos (left p... More about this image found in Oikopleura dioica has postplasmic RNAs . (A-E) Whole-mount in situ hybri...

Fig. 7. Postplasmic RNAs are localized in the vegetal pole region before fertilization. (A-D) Fluorescent whole-mount in situ hybridization (WISH) of unfertilized eggs. The animal pole was determined by DAPI staining of the egg nuclei (white arrowheads). The localization of PLT1-3 mRNAs (A-C... More about this image found in Postplasmic RNAs are localized in the vegetal pole region before fertilizat...

Fig. 8. Screenshots to indicate how to retrieve the RNA-seq data from ANISEED ( https://www.aniseed.fr/ ). (A) Genome browser. (B) The Public track hub in A (red rectangle) used to retrieve the (C) staged RNA-seq (blue rectangle) and (D) AV RNA-seq datasets (green rectangle). Biological replicat... More about this image found in Screenshots to indicate how to retrieve the RNA-seq data from ANISEED ( htt...

Fig. 1. Schematics of extra-embryonic mesoderm development across species. (A) An intermediate stage of reptilian development displaying the vascularized trilaminar omphalopleure, also known as choriovitelline membrane, and the bilaminar omphalopleure. (B) A later developmental stage in squamate... More about this image found in Schematics of extra-embryonic mesoderm development across species. (A) An ...

Fig. 2. Schematics of in vitro stem cell-based 2D and 3D embryo models showing the cell types involved in their production. (A) Mouse epiblast stem cells (mEpiSCs) differentiate into posterior primitive streak cells (mPPSCs) in the presence of activin A, BMP4, FGF and PI3K inhibitor, and then ... More about this image found in Schematics of in vitro stem cell-based 2D and 3D embryo models showing th...

Labeled neurons in the Ciona CNS, including a prominent motor neuron More about this image found in Labeled neurons in the Ciona CNS, including a prominent motor neuron