Fig. 1. Psi depletion reduces proliferation. (A) Third instar larval wing discs with ser -GAL4 expression of UAS - FUCCI with two alternate Psi RNAi lines, or control, stained using anti-pH3 antibody. (B) Quantification of the proportion of cells undergoing each cell cycle stage ( n >10)... More

Fig. 2. Psi binds multiple genomic regions, including Myc . (A) Psi- and RNA Pol II-binding profiles across the Myc gene in larval wing discs ( sd -GAL4 driver used for targeted Psi-DamID and RNA Pol-DamID), shown as log 2 of the ratio to Dam-only control. (B) Average Pol and Psi binding acr... More

Fig. 3. Significantly altered genes after Psi knockdown in wing discs. (A) Genes with statistically significant altered expression after Psi knockdown at FDR<0.01. Top 50 genes with greatest fold change and smallest P -value are labelled. Myc and stg are highlighted with black square... More

Fig. 4. Psi binds and regulates developmental genes. (A) Intersection of differentially expressed genes after Psi knockdown with genes bound by Psi. (B) MA plot showing only genes bound by Psi (blue) while statistically significant DGE events at FDR<0.01 are shown in red. (C) Fold change an... More

Fig. 5. Stg overexpression rescues impaired growth associated with Psi depletion. (A) Psi and RNA Pol II binding profiles across the stg gene in larval wing discs (log 2 of the ratio to Dam-only control). (B) Adult wings with ser -GAL4 driven knockdown of Psi alone or in combination with ... More

Fig. 6. Repression of tok is required for Psi-dependent cell division. (A) Larval wing discs with ser -GAL4-driven tok overexpression (OE) and/or Psi RNAi, or tok 1 / tok 3 transheterozygotes with or without ser -GAL4 driven Psi RNAi KD stained using anti-pH3 to detect mitosis. (B)... More

Fig. 1. Inception increases the base editing scope through de novo PAM generation. (A) Schematic representation of the one-shot inception approach (simultaneous application of two base editors and two guide RNAs). If the adenosine(s) of NGA, NAA and NAG motifs are contained within the canonica... More

Fig. 2. Inception efficiently introduces loss-of-pigmentation mutations in oca2 . (A) Loss-of-function mutations via inception at the oca2 locus by synonymous de novo PAM generation in step 1 (ABE8e, CAG>CGG=p.A337A) and subsequent non-synonymous editing (ancBE4max, CAG>TAG=p.Q333* an... More

Fig. 3. Inception efficiently introduces locally confined multi-codon changes in kcnh6a . (A) Local multi-codon editing via inception (both non-synonymous; anticipated edits are indicated by black arrows) at the kcnh6a locus to create hypomorphic alleles with ABE8e and two guide RNAs [ kcnh6a... More

Fig. 4. Efficient splice site acceptor removal via inception in rx3 . (A) A splice acceptor site mutation (CAG>CAA, black outlined box) in rx3 via intronic de novo PAM generation ( rx3-step1 guide RNA, ABE8e; GAG>GGG) and subsequent C-to-T splice acceptor site editing [sequence adjus... More

Fig. 1. Post-embryonic depletion of CYK-4 results in sterility and a spermathecal morphogenesis phenotype. (A) Confocal images of control (EV RNAi) and cyk-4(RNAi) adults. The control image shows oocytes (oo), the spermatheca (sp), and a nicely formed uterus (ut) filled with multiple embryos (... More

Fig. 2. cyk-4 RNAi worms show cell division defects in the spermathecal lineage. (A) Maximum intensity projections of the spermatheca in control and cyk-4(RNAi) hermaphrodites carrying the nuclear marker P F55B11.3 ::GFP::H2B. Arrows indicate clustered nuclei. (B) Graph showing the average n... More