..., OCT4 and PRDM14, in mouse embryonic stem cells, epiblast-like cells and primordial germ cell-like cells (PGCLCs). PGCLCs are an important system for elucidating primordial germ cell development in mice. We monitored PRDM14 binding during the specification of PGCLCs, identifying direct targets of PRDM14...

... a conditional deletion system to investigate embryonic patterning and lineage specification in response to loss of Oct4. We first observe ectopic expression of Nanog in Oct4-negative postimplantation epiblast cells. The expression domains of lineage markers are subsequently disrupted. Definitive endoderm...

... of this approach by investigating the function of transcription factors ( OCT4 and T ), cell cycle regulators (cyclin D family members) and epigenetic modifiers ( DPY30 ). Overall, sOPTiKD and sOPTiKO provide a unique opportunity for functional analyses in multiple cell types relevant for the study of human...

... exogenous Oct4, Sox2, Klf4 and c-Myc (OSKM) and endogenous SOX2 and NANOG. iRSCs can be stably maintained at low density. At high density, however, they are induced to enter mesenchymal-epithelial transition (MET), resulting in reprogramming to an iPSC state. Morphological changes through MET correlate...

... ) Competing interests The authors declare no competing financial interests. 30 12 2013 16 9 2014 © 2014. Published by The Company of Biologists Ltd 2014 TGFβ Foxh1 Smad Mesendoderm Morphogenesis Cell fate Oct4 Pou5f1 Xenopus tropicalis Signaling Genomics ChIP-seq RNA...

... is induced by Fgf. Knockdown of Sall4 results in loss of spinal cord marker expression and increased expression of pou5f3.2 ( oct25 ), pou5f3.3 ( oct60 ) and pou5f3.1 ( oct91 ) (collectively, pou5f3 genes), the closest Xenopus homologs of mammalian stem cell factor Pou5f1 ( Oct4 ). Overexpression...

... on the activity of a transcriptional network that is fuelled by the activity of three transcription factors (Nanog, Oct4 and Sox2) and balanced by the repressive activity of Tcf3. Extracellular signals modulate the activity of the network and regulate the differentiation capacity of the cells. Wnt/β-catenin...

...Rodrigo Osorno; Anestis Tsakiridis; Frederick Wong; Noemí Cambray; Constantinos Economou; Ronald Wilkie; Guillaume Blin; Paul J. Scotting; Ian Chambers; Valerie Wilson The transcription factors Nanog and Oct4 regulate pluripotency in the pre-implantation epiblast and in derivative embryonic stem...

... embryos. Tead4 -/- embryos do not express trophectoderm-specific genes, such as Cdx2 , but do express ICM-specific genes, such as Oct4 (also known as Pou5f1 ). Consequently, Tead4 -/- morulae do not produce trophoblast stem cells,trophectoderm or blastocoel cavities, and therefore do not implant...

.... The maintenance of their pluripotency and ability to self-renew has been shown to be governed by the transcription factors Oct4(Pou5f1) and Nanog. Oct4 appears to control cell-fate decisions of ESC in vitro and the choice between embryonic and trophectoderm cell fates in vivo. In non-mammalian vertebrates...

... ) is a maternally and zygotically expressed zebrafish gene that encodes the POU domain transcription factor Pou2, an ortholog of mammalian Oct4/Pou5f1. We show that embryos that are genetically depleted of both maternal and zygotic pou2 function(MZ spg ) exhibit extreme DV patterning defects and, independently...

... of Furin to the extra-embryonic region. Activation of Nodal is also necessary to maintain determinants of pluripotency such as Oct4, Nanog and Foxd3 during implantation, and to stimulate elongation of the egg cylinder, before inducing DVE and germ layer formation. We conclude that Nodal is already...

... proliferation rate and enlarged blastomeres. Moreover, smp was shown to control the expression of the pluripotency-associated oct4/pou5f1 gene. We propose that smp is a novel vertebrate-specific gene needed for cell proliferation and that it is probably associated with the maintenance of a pluripotent state...

... and the trophectoderm (TE) that gives rise to the trophoblast lineage. Commitment to ICM lineage is attributed to the function of the two transcription factors, Oct4 (encoded by Pou5f1 ) and Nanog. However, a positive regulator of TE cell fate has not been described. The T-box protein eomesodermin (Eomes...

... an Oct4 gene, which plays an essential role in control of developmental pluripotency, develop to the blastocyst stage and also die after implantation, because they lack pluripotent embryonic cells. Based on this similarity, we posited that cloned embryos derived from differentiated cell nuclei fail...