.../ Summary:   Drosophila spermathecal secretory lineage uses different strengths of Notch signaling that are activated by different ligand use and regulated by different endocytic mechanisms. Notch signaling strength Asymmetric Notch activation Numb Rab5 Deltex University of Connecticut...

... cells and to both supporting and interstitial cell lineages, implying that cells in the CE domain are multipotent progenitors, and suggesting that an asymmetric division is involved in the acquisition of gonadal cell fates. We found that NUMB is asymmetrically localized in CE cells, suggesting...

...Yingshi Ouyang; Claudia Petritsch; Hong Wen; Lily Jan; Yuh Nung Jan; Bingwei Lu Drosophila neuroblasts have served as a model to understand how the balance of stem cell self-renewal versus differentiation is achieved. Drosophila Numb protein regulates this process through its preferential...

..., LGN (Gpsm2) and NuMA, and the cell fate determinant Numb are asymmetrically localized in embryonic lung distal epithelium. Interfering with the function of these proteins in vitro randomizes spindle orientation and changes cell fate. We further show that Eya1 protein regulates cell polarity, spindle...

...Mark Rebeiz; Steven W. Miller; James W. Posakony The Notch cell-cell signaling pathway is used extensively in cell fate specification during metazoan development. In many cell lineages, the conditional role of Notch signaling is integrated with the autonomous action of the Numb protein, a Notch...

... on Notch signaling, it is not necessarily dependent on numb , a gene classically involved in biasing Notch activation. When Numb was removed at the start of larval neurogenesis, both A and B hemilineages were still generated, but by the start of the third larval instar, the removal of Numb resulted in all...

...Suewei Lin; Sen-Lin Lai; Huang-Hsiang Yu; Takahiro Chihara; Liqun Luo; Tzumin Lee Numb can antagonize Notch signaling to diversify the fates of sister cells. We report here that paired sister cells acquire different fates in all three Drosophila neuronal lineages that make diverse types of antennal...

.... Antagonistic interactions between the Notch pathway and the intrinsic cell-fate determinant Numb appear to regulate asymmetric divisions in flies and vertebrates. During these divisions, productive Notch signaling requires sanpodo , which encodes a novel transmembrane protein. Here, we demonstrate...

... at this stage appears to be mediated by the transient expression of Numb in the hemangioblast-derived blast cell colonies. Activation of the Notch pathway was found to inhibit primitive erythropoiesis efficiently through the upregulation of inhibitors of the Wnt pathway. Together, these findings demonstrate...

...,and influences endoderm specification. To gain further insight into how the Notch signaling pathway is regulated during these cell specification events,we identified a sea urchin homologue of Numb (LvNumb). Previous work in other model systems showed that Numb functions as a Notch signaling pathway antagonist...

... to be important for asymmetric segregation of fate-determinants, such as Numb. Here,we show that an intermediate filament protein, transitin, colocalizes with Numb in the cell cortex of mitotic neuroepithelial cells, and that transitin anchors Numb via a physical interaction. Detailed immunohistological and time...

...Martha J. Lundell; Hyung-Kook Lee; Ernesto Pérez; Linda Chadwell Apoptosis is prevalent during development of the central nervous system(CNS), yet very little is known about the signals that specify an apoptotic cell fate. In this paper, we examine the role of Numb/Notch signaling...

... differentiate without further divisions. Interestingly, the progenitors of asymmetric cell lineages adopt a myocardial cell fate as opposed to a pericardial fate when they are unable to divide. These progenitors adopt a pericardial cell fate,however, when cell division is blocked in numb mutants or in embryos...

... asymmetrically distribute the cell-fate determinant Numb to the two daughter cells. Here, we test the possibility that such asymmetric divisions contribute to retinal cell diversification. We have used long-term videomicroscopy of green-fluorescent-protein (GFP)-labeled retinal explants from neonatal rats...

... that NUMB, a NOTCH antagonist, is asymmetrically segregated when some undifferentiated crest-derived cells in nascent dorsal root ganglia undergo mitosis. We conclude that neuron-glia fate determination of crest cells is regulated, at least in part, by NOTCH-mediated lateral inhibition among crest-derived...

... along extending axons. We propose that mechanosensory organ glial cells, the origin of which was until now unknown, are generated by the asymmetric division of pIIb cells. Both Numb and Prospero segregated specifically into the basal glial and neuronal cells during the pIIb and pIIIb divisions...

..., the founders of a dorsal body wall muscle (DA1). In addition to zfh-1 , mesodermal eve itself appears to be needed for correct EPC differentiation, possibly as a direct target of zfh-1 . Epistasis experiments show that zfh-1 specifies EPC development independently of numb , the lineage gene that controls DA1...

...) signaling interacts with the asymmetrically localized Numb (Nb) to specify sibling neuronal fates to daughter cells of GMC-1. In this current study, we have investigated asymmetric cell fate specifications by N and Nb in the context of cell cycle. We have used loss-of-function mutations in N and nb , cell...

... Sensory organ Cell lineage Machrochaetae Microchaetae Prospero Notch Numb Drosophila The mechanosensory bristles on the notum of the adult fly have been utilized extensively to study both pattern formation and cell fate specification ( Jan and Jan, 1993 ). The microchaetae are arranged...

... precursor cells to generate two identical daughter neurons, instead of a neuron and sibling cell. This phenotype is similar to that observed when Notch function is lost late in embryonic development and opposite to the numb loss-of-function phenotype. Genetic interaction studies show that sanpodo...