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Keywords: Nuclear transfer
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Journal Articles
Journal Articles
Journal: Development
Development (2020) 147 (15): dev190777.
Published: 7 August 2020
... developmental potential of these RRF-reduced oocytes with MII-spindle shrinkage was not lost after fertilization. When somatic cell nuclear transfer was performed, the successful generation of cloned mice was somewhat improved and abnormalities were reduced when oocytes with slightly reduced RRF levels were...
Includes: Supplementary data
Journal Articles
Journal: Development
Development (2015) 142 (23): 4049–4055.
Published: 1 December 2015
... and H3K9me3 levels in mouse oocytes and early embryos provides insights into the dynamics of Xist repression and activation during early development and reprogramming. Xist Imprinted XCI Chromatin condensation Oogenesis Histone methylation Nuclear transfer Expression of the large non...
Includes: Supplementary data
Journal Articles
Journal: Development
Development (2013) 140 (12): 2468–2471.
Published: 15 June 2013
... by which reprogramming may be experimentally performed: nuclear transfer to eggs or oocytes, cell fusion, extract treatment, direct reprogramming to pluripotency and transdifferentiation. * Author for correspondence ( rph32@cam.ac.uk ) Competing interests statement The authors declare...
Journal Articles
Journal: Development
Development (2012) 139 (23): 4330–4340.
Published: 1 December 2012
... through the exposure of nuclei to a GV cytoplasmic extract prior to somatic cell nuclear transfer (SCNT) to enucleated oocytes. Here, we developed an extract of GV stage pig oocytes (GVcyto-extract) to investigate epigenetic reprogramming events in treated somatic cell nuclei. This extract induced...
Includes: Supplementary data
Journal Articles
Journal: Development
Development (2010) 137 (17): 2841–2847.
Published: 1 September 2010
... genome. Here, we show that parthenogenetic mouse embryonic cell nuclei can be reprogrammed by serial rounds of nuclear transfer without using any genetic modification. The durations of survival in uteri of cloned foetuses derived from green fluorescent protein (GFP)-labelled parthenogenetic cell nuclei...
Includes: Multimedia, Supplementary data
Journal Articles
Journal: Development
Development (2008) 135 (23): 3935–3945.
Published: 1 December 2008
...-dong, Gwangjin-gu,Seoul 143-701, Korea. † Present address: School of Biology-Oriented Science and Technology, Kinki University, Wakayama 649-6493, Japan. 23 9 2008 © 2008. 2008 Nuclear transfer Chromatin remodeling Histone acetylation Histone methylation Oocyte cytoplasm...
Includes: Supplementary data
Journal Articles
Journal: Development
Development (2006) 133 (8): 1597–1607.
Published: 15 April 2006
...Alice Jouneau; Qi Zhou; Anne Camus; Vincent Brochard; Linda Maulny; Jérôme Collignon; Jean-Paul Renard In mammals, cloning by nuclear transfer (NT) into an enucleated oocyte is a very inefficient process, even if it can generate healthy adults. We show that blastocysts derived from embryonic stem...
Journal Articles
Journal: Development
Development (2004) 131 (10): 2269–2280.
Published: 15 May 2004
... Methylation Nuclear transfer Mammalian genomes with different parental origins are asymmetric in terms of their functions. This asymmetry comprises two biologically important phenomena. First, genomic imprinting causes some genes to be expressed in a manner that is dependent on parental origin, i.e...
Journal Articles
Journal Articles
Journal: Development
Development (1995) 121 (3): 779–783.
Published: 1 March 1995
...Yoko Kato; Yukio Tsunoda ABSTRACT Chimeric embryos between fertilized eggs from F 1 (C57BL×CBA) and 15.5-16.5 days post coitum (dpc) male fetal germ cells (FGCs) from CD-1 strain (glucose phosphate isomerase, Gpi-1a/a) mice were produced by nuclear transfer. Briefly, a single FGC was fused with enu...
Journal Articles
Journal: Development
Development (1991) 113 (2): 561–568.
Published: 1 October 1991
... nuclear transfer nuclear transplantation genomic imprinting protein synthesis Successful development of mammalian embryos requires the presence of both paternal and maternal genomes. Previous studies have revealed that embryos possessing two paternally derived pronuclei (andro-genones) or two...
Journal Articles
Journal Articles
Journal: Development
Development (1988) 102 (4): 773–779.
Published: 1 April 1988
... 1988 nuclear transfer electrofusion parthenote androgenote imprinting mouse embryo Over the past three years, nuclear transfer experiments have firmly established that the participation of both male and female gamete nuclei is essential for full-term development of mouse embryos...
Journal Articles
Journal: Development
Development (1986) 97 (Supplement): 277–289.
Published: 1 October 1986
...James Mcgrath; Davor Solter Copyright © 1986 by Company of Biologists 1986 nucleocytoplasmic interactions mouse embryo transplantation nuclear transfer interspecific haploid T hp mutation parthenogenones Fertilized mammalian ova consist of haploid genomes derived from...