...Jamie Ho; Leslie A. Guerrero; Diana E. Libuda; G. W. Gant Luxton; Daniel A. Starr ABSTRACT Successful nuclear migration through constricted spaces between cells or in the extracellular matrix relies on the ability of the nucleus to deform. Little is known about how this takes place in vivo . We...

... deformation. Here, we establish an in vivo model in which nuclei can be visualized while moving through constrictions and use it to elucidate mechanisms for nuclear migration. C. elegans hypodermal P-cell larval nuclei traverse a narrow space that is about 5% their width. This constriction is blocked...

...Marina Meyerzon; Heidi N. Fridolfsson; Nina Ly; Francis J. McNally; Daniel A. Starr Intracellular nuclear migration is essential for many cellular events including fertilization, establishment of polarity, division and differentiation. How nuclei migrate is not understood at the molecular level...

... of photoreceptor nuclear position depends on a balance of plus-end and minus-end directed microtubule motor function. * Author for correspondence (e-mail: [email protected] ) 14 7 2004 © 2004. 2004 Nuclear migration Dynein Capping protein Dynamitin Kinesin Neurons are highly...

...Daniel A. Starr; Greg J. Hermann; Christian J. Malone; William Fixsen; James R. Priess; H. Robert Horvitz; Min Han Nuclear migration plays an essential role in the growth and development of a wide variety of eukaryotes. Mutations in unc-84, which encodes a conserved component of the nuclear...

... 2000 by Company of Biologists 2000 Oogenesis TGFβ EGF Nuclear migration Follicle cell patterning Broad Complex kekkon pipe Pathway synergy The anterior-posterior (AP) and dorsal-ventral axes (DV) of the Drosophila egg and embryo are established during oogenesis by two...

...Christian J. Malone; William D. Fixsen; H. Robert Horvitz; Min Han ABSTRACT Nuclear migrations are essential for metazoan development. Two nuclear migrations that occur during C. elegans development require the function of the unc-84 gene. unc-84 mutants are also defective in the anchoring...

... head skeleton and a narrowing of the ventral denticle belts. This phenotype could be scored clearly despite the fact that all embryos have posterior segmentation defects. [The latter are an independent phenotype of sqh -A21-derived eggs, probably caused by a failure of axial nuclear migration...

..., and a general deficit and disorder of retinal cells. We have characterized the developmental phenotype in Glued 1 and found defects in multiple stages of eye development, including mitosis, nuclear migration, cell fate determination, rhabdomere morphogenesis and cell death. Transgenic flies that express...

... and cell bodies rise apically and then settle into position in the cell group. The final nuclear positions determine the shape of the individual cells. We have identified a Drosophila gene called marbles which is required for the apical nuclear migrations that accompany cell determination during eye...

..., the mutants exhibited retarded nuclear migration while synchronous nuclear divisions were unaffected. The retarded migration causes nuclei to remain in the anterior part of the embryo retaining their spherical distribution even in a late cleavage stage. This consequently results in an extreme delay in nuclear...