... that BAF localization at the nuclear envelope is eliminated in flies with mutations of the linker of nucleoskeleton and cytoskeleton (LINC) complex in which the LEM-domain protein Otefin is excluded, or after disruption of the nucleus-sarcomere connections. Furthermore, BAF localization at the nuclear...

... to the destabilization of both the nuclear dense lamina (NDL) and the junction between the acroplaxome and the nuclear envelope. Consequently, the acrosome and the manchette fail to be linked to the nucleus leading to the disruption of vesicular trafficking, failure of sperm nuclear shaping and eventually...

...Bernhard Hampoelz; Yannick Azou-Gros; Roxane Fabre; Olga Markova; Pierre-Henri Puech; Thomas Lecuit Nuclear shape is different in stem cells and differentiated cells and reflects important changes in the mechanics of the nuclear envelope (NE). The current framework emphasizes the key role...

...George Dialynas; Sean Speese; Vivian Budnik; Pamela K. Geyer; Lori L. Wallrath The inner side of the nuclear envelope (NE) is lined with lamins, a meshwork of intermediate filaments that provides structural support for the nucleus and plays roles in many nuclear processes. Lamins, classified...

... reprogramming. Consistent with this interpretation, when one of the pro-nuclear envelopes was disrupted by micromanipulation, a modest amount of Brg1 was released into the cytoplasm. However, the broken pro-nucleus still retained significant quantities of this protein ( n =5/5; Fig. 6 ). Our findings...

... with the kinesin-1 light chain KLC-2, as identified in a yeast two-hybrid screen and confirmed by in vitro assays. UNC-83 interacts with and recruits KLC-2 to the nuclear envelope in a heterologous tissue culture system. Additionally, analysis of mutant phenotypes demonstrated that both KLC-2 and the kinesin-1...

...Ya-Hui Chi; Lily I. Cheng; Tim Myers; Jerrold M. Ward; Elizabeth Williams; Qin Su; Larry Faucette; Jing-Ya Wang; Kuan-Teh Jeang The inner nuclear envelope (NE) proteins interact with the nuclear lamina and participate in the architectural compartmentalization of chromosomes. The association of NE...

...Tatiana V. Cohen; Ourania Kosti; Colin L. Stewart MAN1 is an integral protein of the inner nuclear membrane of the nuclear envelope (NE). MAN1 interacts with SMAD transcription factors, which in turn are regulated by the Transforming growth factor beta (TGFβ) superfamily of signaling molecules...

... to be associated with the nuclear envelope, and to be involved in both nuclear anchorage and migration. We investigated the mechanism and function of nuclear anchorage in skeletal muscle cells by generating mice with single and double-disruption of the KASH-domain-containing genes Syne1 (also known as Syne-1...

... ), a new regulator of nuclear morphogenesis and of apical nuclear anchoring. In char -depleted embryos, the nuclei fail to maintain their elongated shape and, instead,become rounded. In addition, together with a disruption of the centrosome-nuclear envelope interaction, the nuclei lose their regular apical...

... in a lethal failure of nuclear envelope assembly in early zygotic divisions. Phenotypes resulting from eyc mutations provide the first in vivo evidence for a role for p47 in membrane biogenesis. Movies available on-line *Author for correspondence (e-mail: dready@bilbo.bio.purdue.edu ) 2 10 2001...

... or the structure of the nuclear lamina of migrating nuclei. We also show that unc-83 encodes a novel transmembrane protein. We identified three unc-83 transcripts that are expressed in a tissue-specific manner. Antibodies against UNC-83 co-localized to the nuclear envelope with lamin and UNC-84. Unlike UNC-84, UNC...