...Steven A. Harvey; Stefan Tümpel; Julien Dubrulle; Alexander F. Schier; James C. Smith During early zebrafish development the nodal signalling pathway patterns the embryo into three germ layers, in part by inducing the expression of no tail ( ntl ), which is essential for correct mesoderm formation...

... Limb Pectoral fin Somites Spadetail No tail Neckless Zebrafish Vertebrate limbs arise bilaterally from the lateral plate mesoderm (LPM) of the embryo. Within the trunk LPM, interactions between the limb bud mesenchyme and the overlying ectoderm result in the initiation of local patterning...

...). † Author for correspondence (e-mail: eisen@uoneuro.uoregon.edu ) * Present address: Department of Anatomy, Cambridge University, Downing Street, Cambridge CB2 3DY, UK 12 11 2003 © 2004. 2004 Somites Motoneurons MiP CaP Zebrafish Spinal cord Trilobite Knypek No tail...

... posterior mesoderm. Thus, fgf8 and fgf24 are together required to promote posterior mesodermal development. We provide both phenotypic and genetic evidence that these Fgf signaling components interact with no tail and spadetail , two zebrafish T-box transcription factors that are required...

.... A. References Amacher, S. L., Draper, B. D., Summers, B. and Kimmel, C. B. ( 2002 ). The zebrafish T-box genes no tail and spadetail are required for development of trunk and tail mesoderm and medial floor plate. Development 129 , 3311 -3323. Barber, C. B., Dobkin, D. P. and Huhdanpaa, H. T. ( 1996...

... in the endoderm from blastula stages and show that its transcription is induced by signals originating from the yolk cell. These signals also induce the mesoderm-expressed transcription factor no tail ( ntl ), whose initial expression coincides with gata5 in the cells closest to the blastoderm margin...

...Kevin J. P. Griffin; Sharon L. Amacher; Charles B. Kimmel; David Kimelman ABSTRACT Inhibition of fibroblast growth factor (FGF) signaling prevents trunk and tail formation in Xenopus and zebrafish embryos. While the T-box transcription factor Brachyury (called No Tail in zebrafish) is a key...

... no tail Brachyury Genetic mosaic Floor plate Myogenesis In 1924, Spemann and Mangold demonstrated that the dorsal blastopore lip of an amphibian gastrula can induce an ectopic embryonic axis when grafted to the ventral side of a host embryo ( Spemann and Mangold, 1924 ). This dorsal tissue...

... with floating head , the zebrafish Xnot homologue, display enhanced floor plate and adaxial muscle phenotypes. Double mutants with no tail (ntl) , the zebrafish homologue of the mouse Brachyury locus, display severe defects in midline and mesoderm formation including absence of most of the somitic mesoderm...

.... Weinberg; Christiane Nüsslein-Volhard ABSTRACT In a large scale screen for mutants with defects in the embryonic development of the zebrafish we identified mutations in four genes, floating head ( flh ), momo ( mom ), no tail ( ntl ), and doc , that are required for early notochord formation. Mutations...

... homologue, no tail ( ntl ), both function as transcription activators. The activation domains of both proteins map to their carboxy terminal regions, and we note that the activation domain is absent in two zebrafish Brachyury mutations, suggesting that it is required for gene function. A dominant...

... ), a gene essential for notochord development. In flh mutants, cells in the notochord domain generate clones of muscle cells. In contrast, notochord domain cells form mesenchyme in embryos mutant for no tail ( ntl ; zebrafish homologue of Brachyury ). A minority of cells in the notochord domain in wild-type...

...S. Schulte-Merker; F. J. M. van Eeden; M. E. Halpern; C. B. Kimmel; C. Nüsslein-Volhard ABSTRACT The mouse T ( Brachyury ) gene is required for normal mesoderm development and the extension of the body axis. Recently, two mutant alleles of a zebrafish gene, no tail ( ntl ), have been isolated...

... the dorsoventral axis of the frog embryo. Here we characterize the zebrafish homologue of goosecoid ( gsc) and compare its expression to that of Brachyury or no tail (ntl) , another immediate early gene required in developing mesoderm. We show that gsc exhibits two indepen- dent phases of expression: an early one...

... and mesodermal derivatives of the head region. Sna-1 expression is induced in animal cap cells by activin A. The early sna-1 expression pattern in gastrulating zebrafish no tail ( ntl ) mutant embryos is normal except a reduction in the level of sna-1 transcription, suggesting that Ntl protein is not the key...

... the gastrula stage, at which time expression is confined to ventral and lateral cells of the marginal zone of the zebrafish embryo. Later, eve1 transcripts are located in the most posterior part of the extending tail tip. We show that LiCl, known to dorsalize Xenopus embryos, has the same effect in zebrafish...

.... In situ hybridization shows that the quantity of snail1 RNA increases at the margin of the blastoderm in cells that involute during gastrulation. As gastrulation begins, snail1 RNA disappears from the dorsal axial mesoderm and becomes restricted to the paraxial mesoderm and the tail bud. snail1 RNA...