... functional properties. However, the underlying molecular mechanism defining these two pacemaker domains remains elusive. Nkx2-5 is a key transcription factor essential for the formation of the working myocardium, but it was generally thought to be detrimental to SAN development. However, Nkx2-5 is expressed...

... (PV) and systemic venous return. The genetic programs that abnormally reinforce pacemaker properties at these sites and how this relates to normal sinoatrial node (SAN) development remain uncharacterized. It was noted previously that Nkx2-5 , which is expressed in the PV myocardium and reinforces...

... to the neonatal stage. Furthermore, we showed that single-cell expression assays coupled with time-lapse microscopy can resolve the identity and the lineage relationships between progenies of single cultured CPCs. With this approach, we found that mESC-derived Nkx2-5 + CPCs preferentially become SMCs or CMs...

...Linda Cambier; Markus Plate; Henry M. Sucov; Mohammad Pashmforoush A complex regulatory network of morphogens and transcription factors is essential for normal cardiac development. Nkx2-5 is among the earliest known markers of cardiac mesoderm that is central to the regulatory pathways mediating...

...David A. Elliott; Mark J. Solloway; Natalie Wise; Christine Biben; Mauro W. Costa; Milena B. Furtado; Martin Lange; Sally Dunwoodie; Richard P. Harvey Homeodomain factor Nkx2-5 is a central component of the transcription factor network that guides cardiac development; in humans, mutations in NKX2.5...

..., 10 mmol/l glutamine, 100 units/ml penicillin and streptomycin (GibcoBRL) in 1% agarose-coated wells for 24 hours with or without recombinant human Heregulin β2 (10 -9 mol/l) ( Fiddes et al.,1995 ). Tbx20 lacZ/+ mice were generated by Ozgene Pty Ltd(Perth, Australia). Nkx2-5 lacZ mice...

... 14 12 2004 ©2005. 2005 Tbx5 Gata4 Nkx2-5 Smooth muscle Transgenesis Knowledge of the regulatory mechanisms underlying cardiac development is essential for understanding the molecular basis of congenital heart defects. During embryonic heart development, differentiation...

... gene Nkx2-5 serves as a marker for spleen precursor tissue. Pre-splenic tissue is initially located in symmetric domains on both sides of the embryo but, during subsequent development, only the left side goes on to form the mature spleen. Therefore, the final location of the spleen on the left side...

...James M. Reecy; Xuyang Li; Miho Yamada; Francesco J. DeMayo; Craig S. Newman; Richard P. Harvey; Robert J. Schwartz Nkx2-5 marks the earliest recognizable cardiac progenitor cells, and is activated in response to inductive signals involved in lineage specification. Nkx2-5 is also expressed...

...Ching-Ling Lien; Chuanzhen Wu; Brian Mercer; Robert Webb; James A. Richardson; Eric N. Olson ABSTRACT The homeobox gene Nkx2-5 is the earliest known marker of the cardiac lineage in vertebrate embryos. Nkx2-5 expression is first detected in mesodermal cells specified to form heart at embryonic day...

... homeobox gene tinman (tin) is expressed in cardiac and visceral mesodermal progenitors and is essential for their specification. In vertebrates, the tin homologue Nkx2-5/Csx and related genes are expressed in early cardiac and visceral mesodermal progenitors. To test for an early cardiogenic function...

... in the adult heart, with only trace levels in skeletal muscle. During murine embryogenesis, endogenous carp expression was first clearly detected as early as E8.5 specifically in heart and is regulated temporally and spatially in the myocardium. Nkx2-5 , the murine homologue of Drosophila gene tinman...