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Keywords: Neurosphere
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Journal Articles
Journal: Development
Development (2006) 133 (4): 675–684.
Published: 15 February 2006
... and investigated their functional properties in vitro. Neurospheres cultured from the adult mouse SVZ in the presence of epidermal growth factor and fibroblast growth factor 2 expressed the ecto-nucleotidases NTPDase2 and the tissue non-specific isoform of alkaline phosphatase, hydrolyzing extracellular ATP...
Journal Articles
Journal: Development
Development (2004) 131 (14): 3433–3444.
Published: 15 July 2004
... of cells expressing high levels of β1 remains. Using neurospheres –aggregate cultures, derived from single stem cells, that have a three-dimensional architecture that results in the localisation of the stem cell population around the edge of the sphere – we show directly thatβ1 integrins are expressed...
Includes: Supplementary data
Journal Articles
Journal: Development
Development (2004) 131 (14): 3423–3432.
Published: 15 July 2004
...-linked anti-rabbit at 1/2000 (Amersham). After 3-7 days culture, neurospheres were washed with cell wash buffer (50 mM Tris-HCl pH 7.5, 0.15 M NaCl, 1 mM CaCl 2 , 1 mM MgCl 2 ) and lysed on ice for 30 minutes in extraction buffer (cell wash buffer plus 0.5% NP40, 2 mM PMSF, 5 μg/ml leupeptine, 2 μg/ml...
Journal Articles
Journal: Development
Development (2002) 129 (1): 233–244.
Published: 01 January 2002
... ( Rubenstein et al., 1994 ; Lumsden and Krumlauf, 1996 ; Wullimann and Puelles, 1999 ). Emx1 Dlx2 En1 Dlx5 Neurosphere Mouse Cell fate © 2002. 2002 2 10 2001 ‡Author for correspondence (e-mail: derek.van.der.kooy@utoronto.ca ) Although the above experiments...