... as permanent mutations in cellular genomes for extraction via single-cell transcriptomics. CRISPR Notch signaling Barcoding Brain development Brain scRNA-seq Neurogenesis National Science Foundation http://dx.doi.org/10.13039/100000001 DGE-2236662 National Institutes of Health...

...Tim J. Yeung; David G. Wilkinson ABSTRACT In the vertebrate nervous system, neurogenesis generally precedes gliogenesis. The mechanisms driving the switch in cell type production and generation of the correct proportion of cell types remain unclear. Here, we show that Fgf20 signalling patterns...

...Roberta Azzarelli; Sarah Gillen; Frances Connor; Jethro Lundie-Brown; Francesca Puletti; Rosalind Drummond; Ana Raffaelli; Anna Philpott ABSTRACT The proneural transcription factor ASCL1 regulates neurogenesis and drives somatic cell reprogramming into neurons. However, not all cell types can...

...: BirthSeq allows the isolation and investigation of alive cells according to their birthdate, in any kind of tissue and vertebrate species. Neurogenesis Neocortex Birds Reptiles FACS sorting Single-cell RNA sequencing Progenitor cells Pallium In situ sequencing Ikerbasque, Basque...

... Her6 gene expression at single-cell level, resulting in rescued early development and a mild phenotype at late stages. Zebrafish Telencephalon Her6 Oscillations Neurogenesis Wellcome Trust http://dx.doi.org/10.13039/100010269 102171/Z/13/Z 224394/Z/21/Z 201380/Z/16/Z 108867/Z...

... how gene expression oscillations encode temporal information during vertebrate embryonic development by discussing the gene expression oscillations occurring during somitogenesis, neurogenesis, myogenesis and pancreas development. These oscillations play important but varied physiological functions...

... developmental stages: in the early hindbrain, transient Fgf3 and Fgf8 signalling from rhombomere 4 is required for correct segmentation, whereas later, neuronal Fgf20 expression confines neurogenesis to specific spatial domains within each rhombomere. How the switch between these two signalling regimes...

... unrestricted use, distribution and reproduction in any medium provided that the original work is properly attributed. Summary: Notch signaling promotes the neurogenesis of late-born cortical fates in mouse brain by upregulating the microRNAs let-7, miR-99a/100 and miR-125b. Cortical development...

... to demonstrate that EIF4A3 promotes cortical development by controlling progenitor mitosis, cell fate and survival. Eif4a3 haploinsufficiency in mice causes extensive cell death and impairs neurogenesis. Using Eif4a3 ; p53 compound mice, we show that apoptosis has the most impact on early neurogenesis, while...

..., thalamus; ve, ventricle. The role of her4 during neural plate stages was reported to be similar to Notch-dependent Drosophila E(spl) genes, as both mediate repression of neurogenesis in the neuroepithelium by lateral inhibition ( Nakao and Campos-Ortega, 1996 ; Takke et al., 1999 ). We found...

...Yun-Ling Hsiao; Hui-Wen Chen; Kuan-Han Chen; Bertrand Chin-Ming Tan; Chia-Hsiang Chen; Haiwei Pi ABSTRACT Neurogenesis is initiated by basic helix-loop-helix proneural proteins. Here, we show that Actin-related protein 6 (Arp6), a core component of the H2A.Z exchange complex SWR1, interacts...

...Neda Masoudi; Ralf Schnabel; Eviatar Yemini; Eduardo Leyva-Díaz; Oliver Hobert ABSTRACT Are there common mechanisms of neurogenesis used throughout an entire nervous system? We explored to what extent canonical proneural class I/II bHLH complexes are responsible for neurogenesis throughout...

... defects, spontaneous seizures and premature death. Loss of UBE4B in the brains of mutant mice results in depletion of neural precursor cells and impairment of neurogenesis. Mechanistically, UBE4B polyubiquitylates and degrades KLHL22, an E3 ligase previously shown to degrade the GATOR1 component DEPDC5...

... ability. Because the NPC pool at the onset of neurogenesis crucially affects the final number of each type of neuron, tight regulation is necessary for the transitional timing from the expansion to the neurogenic phase in these cells. However, the molecular mechanisms underlying this transition are poorly...

... is unknown. We show that the amount of mature miR-9, a key regulator of neuronal development, increases during zebrafish neurogenesis in a sharp stepwise manner. We characterize the spatiotemporal profile of seven distinct microRNA primary transcripts (pri-mir)-9s that produce the same mature miR-9 and show...

... expression profiles of these cells suggest that a niche of laminin 511 and WNT signalling is sufficient to maintain their capability to undergo typical hippocampal neurogenesis. Moreover, laminin 511 signalling sustains the expression of a set of genes responsible for the maintenance of a hippocampal...

... retinogeneis. VSX2 Chromatin Super-enhancers Retina Neurogenesis Bipolar cells Cell fate PAX6 University of Pittsburgh http://dx.doi.org/10.13039/100007921 Washington University in St Louis University of Pittsburgh http://dx.doi.org/10.13039/100007921...

... Human neural progenitor Neurogenesis Diffusion barrier Organoid Cell division ER membrane Summary: Asymmetric segregation of cellular components, which may contribute to the fate and behavior of human neural progenitors, is allowed by a diffusion barrier in the endoplasmic reticulum...

...: The identification of unique molecular markers of GABAergic neuron subtypes in the periaqueductal grey and midbrain reticular formation can facilitate studying the function of specific neuronal circuits. Midbrain reticular formation Deep mesencephalic nucleus Periaqueductal gray Neurogenesis Transcription...

... a subset of cyclins acting at the G1 and S phases of the cell cycle . Mol. Cell. Biol. 32 , 2596 - 2607 . 10.1128/MCB.06745-11 Lange , C. , Huttner , W. B. and Calegari , F. ( 2009 ). Cdk4/cyclinD1 overexpression in neural stem cells shortens G1, delays neurogenesis...