... ) and -dorsomedial (VMH DM ) subdomains, and resulted in a VMH cell fate change from glutamatergic to GABAergic. We observed a loss of Neurog3 expression in Ascl1 −/− hypothalamic progenitors and an upregulation of Neurog3 when Ascl1 was overexpressed. We also demonstrated a glutamatergic to GABAergic fate switch...

...Léon van Gurp; Mauro J. Muraro; Tim Dielen; Lina Seneby; Gitanjali Dharmadhikari; Gerard Gradwohl; Alexander van Oudenaarden; Eelco J. P. de Koning; Allon Klein; Barbara Treutlein ABSTRACT During pancreatic development, endocrine cells appear from the pancreatic epithelium when Neurog3-positive...

... of pancreatic endocrine progenitor induction, specification and segregation using a novel Ngn3 reporter mouse line reveals signature genes that define lineage restriction towards specific endocrine subtypes. Endocrine progenitor-precursor Neurog3 Single cell RNA sequencing Endocrinogenesis Endocrine...

... that upregulation of endocrine lineage genes in Hes1 −/− embryos was the major defect and, accordingly, early pancreas morphogenesis was normalized, and the ectopic pancreas phenotype suppressed, in Hes1 −/− Neurog3 −/− embryos. In Mib1 mutants, we found a near total depletion of dorsal progenitors, which...

...Jonas Ahnfelt-Rønne; Mette C. Jørgensen; Rasmus Klinck; Jan N. Jensen; Ernst-Martin Füchtbauer; Tye Deering; Raymond J. MacDonald; Chris V. E. Wright; Ole D. Madsen; Palle Serup Neurog3-induced Dll1 expression in pancreatic endocrine progenitors ostensibly activates Hes1 expression via Notch...