...Naoki Hirono; Masakazu Hashimoto; Hiromi Shimojo; Hiroshi Sasaki ABSTRACT In preimplantation embryos, epiblast (EPI) fate specification from the inner cell mass is controlled by the segregation of NANOG and GATA6 expression. TEAD–YAP interaction is activated during EPI formation and is required...

... of Nanog, which precipitates breakdown of the naïve state gene regulatory network. Variability in pERK dynamics results in heterogeneous loss of Nanog and metachronous state transition. Knockdown of Nanog allows exit without ERK activation. However, transition to formative pluripotency does not proceed...

... mutants of nanog (M nanog ) in zebrafish, we demonstrated that Nanog tightly controls translation of maternal mRNA during oogenesis via transcriptional repression of eukaryotic translation elongation factor 1 alpha 1, like 2 ( eef1a1l2 ). Loss of maternal Nanog led to defects of egg maturation, increased...

... or differentiation. This property depends on ERK, which downregulates pluripotency genes such as Nanog . Here, we aimed at identifying repressive histone modifications that would mark Nanog for inactivation in response to ERK activity. We found that the transcription factor ZFP57, which binds methylated DNA...

... think this is unlikely as more distant relatives in the colony will have had more opportunity for dispersal, and are less likely to remain neighbours. Other features of the differentiation response could also be extracted from these time series. Nanog protein levels after 16 h differentiation were...

...Marina Veil; Melanie Anna Schaechtle; Meijiang Gao; Viola Kirner; Lenka Buryanova; Rachel Grethen; Daria Onichtchouk ABSTRACT Nanog has been implicated in establishment of pluripotency in mammals and in zygotic genome activation in zebrafish. In this study, we characterize the development of MZ...

...James A. Gagnon; Kamal Obbad; Alexander F. Schier ABSTRACT The role of the zebrafish transcription factor Nanog has been controversial. It has been suggested that Nanog is primarily required for the proper formation of the extra-embryonic yolk syncytial layer (YSL) and only indirectly regulates...

... sets to determine the regulation of developmental gene expression by cell cycle, lineage, motility and environment. We apply this technology to the regulation of the pluripotency gene Nanog in mouse embryonic stem cells. Our data reveal the diversity of cell and population-level interactions with Nanog...

...Elsa Abranches; Ana M. V. Guedes; Martin Moravec; Hedia Maamar; Petr Svoboda; Arjun Raj; Domingos Henrique Heterogeneous expression of the transcription factor NANOG has been linked to the existence of various functional states in pluripotent stem cells. This heterogeneity seems to arise from...

... conditional genetic deletion. Experiments involving mouse embryos deficient for both maternal and zygotic Oct4 suggest that it is dispensable for zygote formation, early cleavage and activation of Nanog expression. Nanog protein is significantly elevated in the presumptive inner cell mass of Oct4 null embryos...

...Rodrigo Osorno; Anestis Tsakiridis; Frederick Wong; Noemí Cambray; Constantinos Economou; Ronald Wilkie; Guillaume Blin; Paul J. Scotting; Ian Chambers; Valerie Wilson The transcription factors Nanog and Oct4 regulate pluripotency in the pre-implantation epiblast and in derivative embryonic stem...

... on the homeodomain-containing transcription factor Nanog. Compared with other pluripotency-associated genes, however, Nanog shows relatively low sequence conservation. Here, we investigated whether Nanog orthologs have the capacity to orchestrate establishment of pluripotency in Nanog –/– somatic cells. Mammalian...

... is dependent on the expression of Nanog . Pluripotency is conserved in the primitive ectoderm of embryos from mammals and urodele amphibians, and here we report the isolation of a Nanog ortholog from axolotls ( axNanog ). axNanog does not contain a tryptophan repeat domain and is expressed as a monomer...

...Yojiro Yamanaka; Fredrik Lanner; Janet Rossant Primitive endoderm (PE) and epiblast (EPI) are two lineages derived from the inner cell mass (ICM) of the E3.5 blastocyst. Recent studies showed that EPI and PE progenitors expressing the lineage-specific transcriptional factors Nanog and Gata6...

...Shinpei Yamaguchi; Kazuki Kurimoto; Yukihiro Yabuta; Hiroyuki Sasaki; Norio Nakatsuji; Mitinori Saitou; Takashi Tada The pluripotency factor Nanog is expressed in peri-implantation embryos and primordial germ cells (PGCs). Nanog-deficient mouse embryos die soon after implantation. To explore...

... pluripotency factor Nanog in human ESCs and in mouse EpiSCs. Nanog in turn prevents neuroectoderm differentiation induced by FGF signalling and limits the transcriptional activity of the Smad2/3 cascade,blocking progression along the endoderm lineage. This negative-feedback loop imposes stasis in neuroectoderm...

.... The maintenance of their pluripotency and ability to self-renew has been shown to be governed by the transcription factors Oct4(Pou5f1) and Nanog. Oct4 appears to control cell-fate decisions of ESC in vitro and the choice between embryonic and trophectoderm cell fates in vivo. In non-mammalian vertebrates...

.... This is in contrast to mouse embryonic stem cells(MESCs) where addition of leukemia inhibitory factor (LIF) replaces the need for a feeder layer. Recently, the transcription factor Nanog was suggested to allow LIF and feeder-free growth of MESCs. Here, we show that NANOG overexpression in HESCs enables...

... and the trophectoderm (TE) that gives rise to the trophoblast lineage. Commitment to ICM lineage is attributed to the function of the two transcription factors, Oct4 (encoded by Pou5f1 ) and Nanog. However, a positive regulator of TE cell fate has not been described. The T-box protein eomesodermin (Eomes...

.... * Author for correspondence (e-mail: [email protected] ) 10 9 2003 © The Company of Biologists Limited 2003 2003 Microarray Anteroposterior axis Gastrulation Signalling pathways Tdgf1 Nanog Tissue and organ development of vertebrates is tightly regulated by signalling...