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Keywords: Nanobody
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Journal Articles
Journal: Development
Development (2021) 148 (18): dev199822.
Published: 01 September 2021
... techniques. Here, we present a nanobody-based method, Nanobody Nuclear Trap (NaNuTrap), which works with the existing Gal4/UAS system in Drosophila and allows for early in vivo cell nuclei labelling independently of the maturation time of the fluorescent protein. This restores the utility of fluorescent...
Includes: Supplementary data
Journal Articles
Journal: Development
Development (2018) 145 (2): dev148874.
Published: 26 January 2018
... as versatile tools that can be used to target and manipulate proteins. Such protein binders can be based on various scaffolds, such as nanobodies, designed ankyrin repeat proteins (DARPins) and monobodies, and can be used to block or perturb protein function in living cells. In this Primer, we provide...
Journal Articles
Journal: Development
Development (2015) 142 (10): 1879–1884.
Published: 15 May 2015
... provided that the original work is properly attributed. SUMMARY: Chromobodies - small, intracellular fluorescent antibodies - are used to trace endogenous antigens, without the need for direct protein tagging, in zebrafish embryos. Actin PCNA Live imaging Nanobody Protein dynamics...
Includes: Supplementary data