.... Endocytic activity gradually reduces as tissue remodeling progresses. This reduced endocytic activity accelerates cell elimination through the regulation of Myosin II subcellular reorganization, junctional E-cadherin levels, and caspase activation. Whereas the increased Myosin II dynamics accelerates cell...

..., suggesting that the junctional network relaxes to accommodate germline growth. At that time, a prominent medial Myosin II network coupled to corrugating adherens junctions develops. Local enrichment of medial Myosin II in main body follicle cells resists germline-derived forces, thus constraining apical...

... activation of myosin-II, which becomes localized to the apical domains of cells just prior to rosette formation ( Ernst et al., 2012 ; Harding and Nechiporuk, 2012 ). Myosin-II is activated by phosphorylation, which is dependent on both Rho kinase 2a (Rock2a) and the scaffolding molecule Shroom3; loss...

... that the kinases Rok and Drak function redundantly during Myosin II-dependent cell constriction, subsequent multicellular alignment and AJ remodelling. In addition, we show that early multicellular patterning characterized by cell alignment is promoted by the conserved transcription factor Atonal (Ato). Further...

... and aligns into parallel columns. Live imaging shows that this is accomplished by the shrinkage of select cell interfaces, as three-cell junctions are converted to four-cell junctions. Additionally, we show that non-muscle Myosin II and the polarity proteins Discs large (Dlg) and Bazooka are enriched along...

... apical circumference remodeling failed or was incomplete in all the mouse mutants affected in hair bundle morphogenesis that we tested. During the normal shape transition, myosin VIIa and myosin II (A and B isoforms) displayed polarized redistributions into and out of the developing lobes, respectively...

..., Hybridoma Bank), 1:10; mouse anti-GEF64C ( Bashaw et al.,2001 ) 1:50; rabbit anti-DRhoGEF2( Padash Barmchi et al., 2005 )1:1000; rabbit anti-Myosin II heavy chain( Bloor and Kiehart, 2001 )1:500; mouse anti-Rho1 (P1D9) ( Magie et al., 2002 ) 1:50 and Phalloidin Alexa-594 (Molecular Probes) 1:200. All...

...Change Tan; Beth Stronach; Norbert Perrimon Myosins are a superfamily of actin-dependent molecular motor proteins,among which the bipolar filament forming myosins II have been the most studied. The activity of smooth muscle/non-muscle myosin II is regulated by phosphorylation of the regulatory...

...Victoria E. Foe; Christine M. Field; Garrett M. Odell ABSTRACT We studied cyclic reorganizations of filamentous actin, myosin II and microtubules in syncytial Drosophila blastoderms using drug treatments, time-lapse movies and laser scanning confocal microscopy of fixed stained embryos (including...