... and myod genes, which are required for commitment to myogenesis. Tbx16 activity depends on Fgf signalling from the organiser. In contrast, Tbxta is not required for myf5 expression, but binds a specific site upstream of myod that is not bound by Tbx16 and drives (dependent on Fgf signals) myod expression...

... in the establishment of the muscle phenotype remains elusive. Here, we show that the NFAT member NFATc2 cooperates with the basic helix-loop-helix transcription factor MyoD to induce the expression of a specific myosin heavy chain (MHC) isoform, the neonatal one, during embryogenesis. We found this cooperation...

...Natalia Moncaut; Joe W. Cross; Christine Siligan; Annette Keith; Kevin Taylor; Peter W. J. Rigby; Jaime J. Carvajal The specification of the skeletal muscle lineage during craniofacial development is dependent on the activity of MYF5 and MYOD, two members of the myogenic regulatory factor family...

... and differentiation into fibers, demonstrating that regulation of pioneer patterning is somite-intrinsic. Furthermore, pioneer myoblasts express Robo2 downstream of MyoD and Myf5, whereas the dermomyotome and caudal sclerotome express Slit1. Loss of Robo2 or of sclerotome-derived Slit1 function perturbed both...

... asymmetric cell division in C. elegans. Dev. Cell 11 , 105 -115. Bianchi, M. E. and Agresti, A. ( 2005 ). HMG proteins: dynamic players in gene regulation and differentiation. Curr. Opin. Genet. Dev. 15 , 496 -506. Baugh, L. R. and Hunter, C. P. ( 2006 ). MyoD,modularity, and myogenesis...

...Yaniv Hinits; Daniel P. S. Osborn; Simon M. Hughes Myogenic regulatory factors of the Myod family (MRFs) are transcription factors essential for mammalian skeletal myogenesis. However,the roles of each gene in myogenesis remain unclear, owing partly to genetic linkage at the Myf5/Mrf4 locus...

...Lisa Maves; Andrew Jan Waskiewicz; Biswajit Paul; Yi Cao; Ashlee Tyler; Cecilia B. Moens; Stephen J. Tapscott The basic helix-loop-helix (bHLH) transcription factor Myod directly regulates gene expression throughout the program of skeletal muscle differentiation. It is not known how a Myod-driven...

... inhibition of vessel formation 2 days after grafting, whereas PBS beads did not affect vessel organization( Fig. 6A-D ; n =22 sFLT1 beads; n =14 PBS beads). Analysis of MyoD expression showed reproducible muscle fusion in the dorsal regions of the sFLT1 grafted wings( Fig. 6E-H ), whereas PBS beads did...

... to a shift in mitotic orientation and to fate segregation. Site-directed electroporation to additional, discrete somite regions, further reveals that N-cadherin-mediated adhesion is necessary for maintaining the epithelial configuration of all dermomyotome domains while promoting the onset of Myod...

... at least in part from a lack of myoblast proliferation in the hypaxial muscle domain. Conversely, overexpression of lbx1 mRNA results in enlarged somites, an increase in cell proliferation, but a lack of differentiated muscle. The control of cell proliferation is linked to a strong downregulation of myoD...

...Julie A. Groves; Christina L. Hammond; Simon M. Hughes Fibroblast growth factors (Fgfs) have long been implicated in regulating vertebrate skeletal muscle differentiation, but their precise role(s) in vivo remain unclear. Here, we show that Fgf8 signalling in the somite is required for myod...

.... elegans Myod homolog HLH-1 acts downstream of mls-2 to specify M-derived coelomocyte cell fates. Thus MLS-2 functions in a cell type-specific manner to regulate both cell proliferation and cell fate specification. The proper formation of a complex multicellular organism requires the precise coordination...

... activate hlh-1 in blastomeres that either lack POP-1/TCF or that have down-regulated POP-1/TCF in response to Wnt/MAP kinase signaling. The potent myogenic activity of HLH-1 highlights the remarkable developmental plasticity of early C. elegans blastomeres and reveals the evolutionary conservation of MyoD...

... ; kpatel@rvc.ac.uk ) 26 10 2004 ©2005. 2005 Striated sphincter muscles Cloaca Perineum Chick/quail chimera Somite Segmental origin limbless MyoD Pax3 Pax7 Met Lbx1 Meox2 We thank three anonymous reviewers for comments that significantly improved...

... and differentiation of graft-derived cells were assayed using QCPN and QH1 antibodies to identify all quail cells and quail endothelial cells, respectively. Chimeric embryos were assayed for expression of myf5, myod, paraxis and lbx1 , and the synthesis of myosin heavy chain (MyHC), between 1 and 6 days later (stages...

... 3 2004 © 2004. 2004 Dermomyotome Slow muscle MyoD Pax3 Myf5 Engrailed During vertebrate skeletal muscle development, several populations of myogenic cells arise within each somite due, at least in part, to signals impinging on the somite from neighbouring tissues( Borycki...

... domain is necessary for Mash1 to be able to promote neuronal differentiation, and is sufficient to confer this activity to the non-neural bHLH factor MyoD. In contrast, helix 2 of Math1, and both helix 1 and 2 of Mash1, are the domains required for the neuronal specification activities of these factors...

...Marie-Claire Delfini; Delphine Duprez Forced expression of the bHLH myogenic factors, Myf5 and MyoD, in various mammalian cell lines induces the full program of myogenic differentiation. However, this property has not been extensively explored in vivo. We have taken advantage of the chick model...

... this discrepancy, we have re-addressed this issue, using short-term in vivo microsurgery and culture experiments in chick. Our results show that the initial expression of the muscle-specific markers Myf5 and MyoD is regulated in a mesoderm-autonomous fashion. The reception of a Wnt signal is required for MyoD...

.... Six1 –/– embryos have impaired primary myogenesis, characterized, at E13.5, by a severe reduction and disorganisation of primary myofibers in most body muscles. While Myf5,MyoD and myogenin are correctly expressed in the somitic compartment in early Six1 –/– embryos, by E11.5 MyoD and myogenin gene...