... cells Single cell RNA-seq Muscle regeneration Mouse National Institute of Arthritis and Musculoskeletal and Skin Diseases 10.13039/100000069 AR041126 AR041164 Growth and homeostasis of the skeletal muscle system, a tissue/organ constituting approximately 35% of the human total...

... of the mTOR complexes 1 (mTORC1) and 2 (mTORC2) in mouse muscle progenitors. We show that inactivation of mTORC1, but not mTORC2, in developing muscle causes perinatal death. In the adult, mTORC1 deficiency in muscle stem cells greatly impinges on injury-induced muscle regeneration. These phenotypes...

...James G. Tidball; Kenneth Dorshkind; Michelle Wehling-Henricks Much of the focus in muscle regeneration has been placed on the identification and delivery of stem cells to promote regenerative capacity. As those efforts have advanced, we have learned that complex features of the microenvironment...

... to inhibition of the myogenic program during embryogenesis. Surprisingly however, Dlk1 depletion improves in vitro and in vivo adult skeletal muscle regeneration by substantial enhancement of the myogenic program and muscle function, possibly by means of an increased number of available myogenic precursor cells...

...Christine M. Snyder; Amanda L. Rice; Nelsa L. Estrella; Aaron Held; Susan C. Kandarian; Francisco J. Naya Understanding the molecular mechanisms of skeletal muscle regeneration is crucial to exploiting this pathway for use in tissue repair. Our data demonstrate that the MEF2A transcription factor...

... that appear during muscle regeneration was finally established. Subsequently, it was demonstrated that, when grafted, satellite cells could also self-renew, conferring on them the coveted status of ‘stem cell’. The emergence of other cell types with myogenic potential, however, questioned the precise role...

... the somite. We hypothesize that guidance signaling might impact multiple steps in muscle regeneration, including escape from the niche, directed migration to sites of injury, cell-cell interactions among satellite cell progeny, and differentiation and patterning of regenerated muscle. * Author...

...Malea M. Murphy; Jennifer A. Lawson; Sam J. Mathew; David A. Hutcheson; Gabrielle Kardon Muscle regeneration requires the coordinated interaction of multiple cell types. Satellite cells have been implicated as the primary stem cell responsible for regenerating muscle, yet the necessity...

... for muscle regeneration. Here, we have engineered genetic ablation of Pax7 + cells to test whether there is any significant contribution to muscle regeneration after acute injury from cells other than this source. We find that such elimination of Pax7 + cells completely blocks regenerative myogenesis either...

... that round cells are satellite-cell descendants with stem cell-like characteristics and represent a useful source of donor cells to improve muscle regeneration. Our findings raise a simple question. Why did many previous studies not describe the stem-like cells that are similar to round cells? Only a few...

... lamina muscle regeneration rat During development, innervation of mammalian skeletal muscle has two major effects on the expression of the acetylcholine receptor (AChR) (reviewed by Schuetze and Role, 1987 ). The first is to suppress the constitutive expression of ‘fetal’ AChRs, which...

... the two proteins is likewise found in regenerating and denervated muscles. Cardiac TnT is re-expressed during muscle regeneration, like embryonic and neonatal MHCs ( Sartore et al . 1982 ) and embryonic myosin light chain ( Carraro et al . 1983 ), and can thus represent a useful marker...