... this transition, transcription factors and chromatin are rewired to encode new functional features. Here, we examine the role of mitogen-activated protein kinase (ERK1/2) signalling in pluripotent state transition. We show that a primary consequence of ERK activation in mouse embryonic stem cells is elimination...

... is controlled by FGF/ERK signaling. However, only a few studies have addressed the dynamic activity of the FGF/ERK signaling network in pluripotent cells at high time resolution. Here, we use live cell sensors in wild-type and Fgf4- mutant mouse embryonic stem cells to measure dynamic ERK activity in single...

... is essential for the HSC generation and, therefore, all derivatives lineages. Using mouse embryonic stem cells (mESCs) to recapitulate murine embryonic development, we have studied the requirement for Notch signaling during the earliest B-cell lymphopoiesis and found that Rbpj -deficient mESCs are able...

... of Gli3 protein. GPR17 also negatively regulates Shh signalling in neural cells differentiated from mouse embryonic stem cells, suggesting that GPR17 function is conserved among different organisms. Our results demonstrate that GPR17 is a novel negative regulator of Shh signalling in a wide range...

... ( [email protected] ) Competing interests The authors declare no competing financial interests. 12 3 2014 4 7 2014 © 2014. Published by The Company of Biologists Ltd 2014 Patched1 Patched2 Shh Genome editing Mouse embryonic stem cells Shh signaling...

...Fernando Faunes; Penelope Hayward; Silvia Muñoz Descalzo; Sujash S. Chatterjee; Tina Balayo; Jamie Trott; Andrew Christoforou; Anna Ferrer-Vaquer; Anna-Katerina Hadjantonakis; Ramanuj Dasgupta; Alfonso Martinez Arias The maintenance of pluripotency in mouse embryonic stem cells (mESCs) relies...