... embryonic cell aggregates are aspired through constrictions in microfluidic channels, generating highly heterogeneous flows and large cell deformations that can be imaged using two-photon microscopy. This approach provides a way to measure in situ local viscoelastic properties of 3D tissues and connect them...

...Matthew J. Stower; Shankar Srinivas ABSTRACT Live imaging is an important part of the developmental biologist's armoury of methods. In the case of the mouse embryo, recent advances in several disciplines including embryo culture, microscopy hardware and computational analysis have all contributed...

...Mostafa Aakhte; Hans-Arno J. Müller ABSTRACT Light-sheet or selective plane illumination microscopy (SPIM) is ideally suited for in toto imaging of living specimens at high temporal-spatial resolution. In SPIM, the light scattering that occurs during imaging of opaque specimens brings about...

.... Style transfer (see Glossary, Box 1 ) has been famously applied in the context of artistic illustrations, allowing any photographs to be turned into van Gogh paintings ( Gatys et al., 2016 ). Similarly, it can be used to learn the image style of different microscopy modalities, with numerous...

... used to image lipid droplets in mammalian oocytes and embryos, but this method is not quantitative and often incompatible with live cell imaging and subsequent development. Here we have applied chemically specific, label-free coherent anti-Stokes Raman scattering (CARS) microscopy to mouse oocytes...

... of genome folding and unravel the regulatory functions of nuclear organization. Nuclear organization Chromosome conformation capture Epigenetics Microscopy Nuclear organization, the physical structure of the genome within the nuclear space, has fascinated cellular and developmental...

... The authors declare no competing financial interests. 2 5 2013 © 2013. Published by The Company of Biologists Ltd 2013 Brainbow Zebrafish Clonal analysis In vivo imaging Lineage Microscopy A deeper understanding of developmental and neurobiological processes...

...Paul J. Scherz; Jan Huisken; Pankaj Sahai-Hernandez; Didier Y. R. Stainier Knowing how mutations disrupt the interplay between atrioventricular valve(AVV) morphogenesis and function is crucial for understanding how congenital valve defects arise. Here, we use high-speed fluorescence microscopy...