... cell populations within the developing Xenopus embryo. * Author for correspondence (e-mail [email protected] ) 10 09 1998 20 10 1998 © 1998 by Company of Biologists 1998 Homeobox genes Heart development Microinjection Xenopus laevis XNkx2-3 XNkx2-5 Tinman...

... Microinjection HRP In the mouse, gastrulation begins approximately midway through embryonic day (E) 6 (for reviews, see Tam and Behringer, 1997 ; Tam et al., 1993 ). At this time, the embryo consists of a single layer of primitive ectoderm (epiblast) covered by a layer of primitive endoderm...

.... * Author for correspondence (e-mail: [email protected] ) 12 02 1998 01 04 1998 © 1998 by Company of Biologists 1998 Cranial neural crest Cell migration Chick Microinjection Fusion protein Whole mount immunostaining Explant culture Pattern formation Cell...

... the normal developmental potential of neural crest cells by performing in situ cell lineage analysis in the mouse by microinjecting lysinated rhodamine dextran (LRD) into individual dorsal neural tube cells in the trunk. Labeled progeny derived from single cells were found in the neural tube, dorsal root...

... and the blastopore was located at the site of the future anus of the nauplius larva. In order to trace cell lineages and determine axial relationships, single 2and 4-cell-stage blastomeres were microinjected with rhodamine-dextran. The results showed that the mesendoderm cells which initiated gastrulation were...

..., 1980 ). There are indications that these granules are lysosomes, which take up exogenous macromolecules by fluid-phase pinocytosis ( Clokey and Jacobson, 1986 ). C. elegans nematode gut differentiation autofluorescence yolk microinjection Lucifer Yellow Rhodamine video enhancement...

... in the population as a whole. 13 08 1991 © 1991 by Company of Biologists 1991 germ layer formation mouse embryo cell fate fate map cell lineage embryo growth gastrulation microinjection HRP The epiblast or primitive ectoderm of the mouse embryo is the sole source of all the fetal...

... of localization. Here, we test the developmental potential of migrating trunk neural crest cells by microinjecting a vital dye, lysinated rhodamine dextran (LRD), into individual cells as they migrate through the somite. By two days after injection, the LRD-labelled clones contained from 2 to 67 cells, which were...

... State University, Terre Haute, Indiana 47809, USA © 1990 by Company of Biologists 1990 microinjection mosaicism RSV-LTR transgenes variegated expression zebrafish The production of transgenic organisms has been of great value in the study of the genetic basis of embry onic...

... transgenic mouse line was produced by microinjection of C57/CBA fertilized egg pronuclei (obtained at day. 0.5 post-coitus) with the 1.95 kb Xhol-Xbal pEIII65- CAT fragment at a concentration of 2 μ gml -1 . Six offspring were obtained from surrogate mothers that received microinjected 1-cell embryos...

... with Gel/mount (Biomedia Corp.) prevented dye spreading and allowed long-term preservation of sections. Slides were viewed immediately on an epifluorescent microscope through a rhodamine filter set to visualize the Dil, and data were recorded photographically. To determine if the microinjection...

...Patrick H. Krone; John J. Heikkila ABSTRACT The expression of microinjected chimeric genes containing Drosophila hsp 70 and Xenopus hsp 70 and hsp 30 promoters linked to the reporter gene coding for bacterial chloramphenicol acetyltransferase (CAT) was examined during early development of Xenopus...

... segments. Such embryos can be ‘rescued’, i.e. restored to normal development, by microinjection of cytoplasm or RNA from unirradiated donor embryos. Most of the rescued embryos look completely normal and many of them hatch spontaneously. The rescuing activity decreases from the anterior to the posterior...

... to the metaphase. We show here that non-hormone-stimulated starfish oocytes, induced to mature by microinjection of MPF-containing cytosol taken from hormone-stimulated oocytes, produce normal bipin-naria larvae once fertilized. The same result was obtained when donor oocytes were induced to mature...

... be integrated into the fish genome, that the resulting transgenic fish are mosaic and that some of these mosaic individuals give rise to stable lines of transgenic fish. 23 02 1988 © 1988 by Company of Biologists 1988 germ-line transgenic zebrafish microinjection mosaicism DNA...

...Roberta R. Franks; Barbara R. Hough-Evans; Roy J. Britten; Eric H. Davidson A method is described for microinjection of cloned DNA into the zygote nucleus of Lytechinus variegatus . Eggs of this species are unusually transparent, facilitating visual monitoring of the injection process. The initial...

...Kirstie A. Lawson; Roger A. Pedersen; Sara Van De Geer ABSTRACT The fate of the embryonic endoderm (generally called visceral embryonic endoderm) of prestreak and early primitive streak stages of the mouse embryo was studied in vitro by microinjecting horseradish peroxidase into single axial...

... and microinjection of heterologous proteins using Hyalophora follicles. By microinjecting fluorescently labelled acidic and basic proteins into the nurse cells or oocyte of vitellogenic Drosophila follicles, we failed to obtain evidence for charge-dependent migration of these molecules. We have also analysed...

... retract within a time span of about 3h. In this case transcription is inhibited by binding of the antibodies to the loop DNA, thereby blocking the progression of the RNA polymerases along the chromatin axis. Interestingly, microinjected DNA-antibodies do not inhibit rDNA tran-scription ( Fig. 6A ) which...

...D. A. Melton; M. R. Rebagliati Copyright © 1986 by Company of Biologists 1986 anti-sense RNA frog egg oocyte microinjection mRNA. In general, it is difficult to identify genes that play critical roles in developmental decisions in vertebrates because it is not possible...