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Keywords: Microarrays
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Journal Articles
Journal: Development
Development (2006) 133 (16): 3147–3157.
Published: 15 August 2006
... together. Strong loss-of-function mutations in either efl-1 or dpl-1 cause defects in oogenesis that result in sterility, while lin-35 mutants are fertile with reduced broods. Microarray-based expression profiling of dissected gonads from efl-1, dpl-1 and lin-35 mutants reveals that EFL-1 and DPL-1 promote...
Includes: Supplementary data
Journal Articles
Journal: Development
Development (2006) 133 (4): 711–723.
Published: 15 February 2006
... tightly controlled genetic programme where specific sets of genes are upregulated. Some of them, for example, control membrane invagination between the nuclei anchored at the apical surface of the syncytium. We used microarrays to describe the global programme of gene expression underlying cellularisation...
Includes: Supplementary data
Journal Articles
Journal: Development
Development (2005) 132 (17): 3947–3961.
Published: 01 September 2005
... extracted by the guanidinium-acid phenol method [Trizol, Invitrogen( Chomczynski and Sacchi,1987 )]. This RNA was used to make double-stranded cDNA and was amplified with the SMART method ( Matz et al., 1999 ) (Clontech). The resulting cDNA was used to synthesise fluorescently labelled microarray probes and...
Journal Articles
Journal: Development
Development (2004) 131 (2): 311–323.
Published: 15 January 2004
... fourth larval stage (L4) or young adult stage. We also collected poly A+ RNA from him-5(e1490) and glp-4(bn2);him-5(e1490) purified adult male populations. Each sample was independently grown and harvested four times. Using DNA microarrays representing 18,010 of the 19,546 protein-encoding genes...
Includes: Supplementary data
Journal Articles
Journal: Development
Development (2003) 130 (25): 6257–6272.
Published: 22 December 2003
... genotype were compared by hybridization to cDNA microarrays. Among the 83 genes differentially expressed, we found genes known to be enriched in FCs or FCMs,such as heartless or hibris , previously characterized genes with unknown roles in muscle development, and predicted genes of unknown function. Our...
Includes: Supplementary data
Journal Articles
Journal: Development
Development (2003) 130 (24): 6001–6012.
Published: 15 December 2003
... microRNAs play an important role in the regulation of flowering. Scanned images were saved as .CEL files using default settings of MicroArray Suite 5.0 (Affymetrix). Numeric values representing the signal of each feature were analyzed using scripts and statistical methods developed by Borevitz and...
Includes: Supplementary data