... microtissues in vitro that integrate both cell types in complex 3D structures. We established conditions for simultaneous differentiation of cardiomyocytes and endothelial cells from human pluripotent stem cells following initial cardiac mesoderm induction. The endothelial cells expressed cardiac markers...

... no competing financial interests. 26 4 2013 18 9 2013 © 2013. Published by The Company of Biologists Ltd 2013 Transcriptional derepression Embryonic axis formation Mesoderm induction Down syndrome Polycomb group proteins Xenopus During amphibian early...

...Stephen A. Green; Rachael P. Norris; Mark Terasaki; Christopher J. Lowe FGFs act in vertebrate mesoderm induction and also play key roles in early mesoderm formation in ascidians and amphioxus. However, in sea urchins initial characterizations of FGF function do not support a role in early mesoderm...

... is essential for tail formation during zebrafish development, the differential regulation of mesoderm induction by BMP signalling during Xenopus and zebrafish development may be central to differences in tail formation. We thank past and present members of the Smith lab for their help and advice...

... of undifferentiated ES cells. Instead,such manipulations restrict the ability of ES cells to commit to differentiation. ES cells lacking Fgf4 or treated with FGF receptor inhibitors resist neural and mesodermal induction, and are refractory to BMP-induced non-neural differentiation. Lineage commitment potential...

... an important role in embryonic patterning by regulating the production of a subset of active mature TGFβ proteins in specific sites. * Author for correspondence (e-mail: [email protected] ) 29 11 2004 ©2005. 2005 PACE4 TGFβ Mesoderm induction Vg1 In Xenopus , the initial...

...Maki Daniels; Kazuya Shimizu; Aaron M. Zorn; Shin-ichi Ohnuma Mesoderm induction and patterning are primarily regulated by the concentration of locally expressed morphogens such as members of the TGFβsuperfamily. Smad2 functions as a transcription factor to regulate expression of mesodermal genes...

.... Here, we show that activin, the function of which in mesoderm induction has hitherto been unclear, also plays a role in mesoderm formation. Inhibition of activin function using antisense morpholino oligonucleotides interferes with mesoderm formation in a concentration-dependent manner and also changes...

... ) 7 1 2004 © 2004. 2004 Mesoderm induction Smad2/3 Axis patterning Nodal Mouse Members of the Transforming Growth Factor β (TGFβ) superfamily of secreted growth factors regulate numerous embryonic processes, from axis formation and mesoderm patterning to allocation...

... members. The work confirms that derrière plays an important role in mesoderm formation and it illustrates the complex regulation to which inducing factors are subject. †Author for correspondence (e-mail: [email protected] ) 2 7 2002 © 2002. 2002 Xenopus Mesoderm induction...

...Peter M. Eimon; Richard M. Harland Derrière is a recently discovered member of the TGFβ superfamily that can induce mesoderm in explant assays and is expressed at the right time and location to mediate mesoderm induction in response to VegT during Xenopus embryogenesis. We show that the ability...

... is a determinant of patterning in the early Xenopus embryo. *Author for correspondence (e-mail: [email protected] ) 16 5 2001 © 2001. 2001 Smad Phosphorylation TGFβ Nodal Mesoderm induction Signal transduction Xenopus laevis In the developing Xenopus embryo...

... and Melton, 1995 ; Schulte-Merker et al., 1994 ), inhibits mesoderm induction and dorsal axis formation ( Hemmati-Brivanlou and Melton, 1992 ). Smad TGFβ Phosphorylation Mesoderm induction Signal transduction Xenopus laevis © 2000 by Company of Biologists 2000 11 04 2000 13...

... B-Vg1 -mediated, but not by eFGF -mediated, mesoderm induction in animals caps. However, wild-type XHNF1 β mRNA does not trigger Xbra expression in animal caps. We conclude that XHNF1 β function is essential, though not sufficient, for mesoderm induction in the Xenopus embryo. * Author...

...Eric Agius; Michael Oelgeschläger; Oliver Wessely; Caroline Kemp; E. M. De Robertis ABSTRACT In Xenopus , mesoderm induction by endoderm at the blastula stage is well documented, but the molecular nature of the endogenous inductive signals remains unknown. The carboxy-terminal fragment of Cerberus...

... regulator of mesoderm induction by ectopic TGFβ superfamily ligands and during endogenous patterning: ectopic expression of mutationally activated FAST-1, ectopic expression of dominant inhibitory FAST-1, and injection of a blocking antibody specific for FAST-1. Expression of constitutively...

....120.7.1919 Ding , X. , Hausen , P. and Steinbesser , H. ( 1998 ) Pre-MBT patterning of early gene regulation in Xenopus : the role of the cortical rotation and mesoderm induction . Mech. Dev . 70 , 15 – 24 . 10.1016/S0925-4773(97)00163-9 Dohrmann , C. E. , Hemmati...

... a dominant negative cleavage mutant Xnr2 (cmXnr2) to carry out loss-of-function experiments to explore the requirement for Xnr signaling in early amphibian embryogenesis, and the relationship between activin and Xnrs. cmXnr2 blocked mesoderm induction caused by Xnr , but not activin , RNA. In contrast...

... Mesoderm induction Cell migration Motility goosecoid Mix . 1 Activin FGF Adhesion Cell polarity Gastrula During amphibian gastrulation, several morphogenetic processes cooperate to transform a simple vesicle-like blastula into a multilayered structure representing the basic body plan...

... hoxa-1 expression is induced (arrowheads) around the transplanted donor cells (brown). Scale bars, 100 μm. Given the potential role for fibroblast growth factors (FGFs) in mesoderm induction ( Kimelman and Kirschner, 1987 ), we blocked the mesoderm formation by the injection of mRNA encoding...