...Shufan Lin; Bomyi Lim ABSTRACT Although fluctuations in transcription factor (TF) dosage are often well tolerated, TF dosage modulation can change the target gene expression dynamics and result in significant non-lethal developmental phenotypes. Using MS2/MCP-mediated quantitative live imaging...

... translational repression of zygotic hunchback mRNAs. Hunchback Drosophila embryo Translation SunTag Live imaging MS2 The gap gene hunchback ( hb ) is essential for body plan specification during Drosophila embryogenesis, primarily by establishing the gene expression boundaries...

... shown are from one representative of three independent experiments. The AX2 cell line was used to facilitate greater temporal homogeneity in development. (C) Live imaging of pspA transcription in intact slugs. 128 MS2 repeats were inserted into the pspA gene. Nascent transcripts were detected...

... – optical transparency during embryogenesis, genetic tractability and rapid development. Therefore, to study transcription and RNA dynamics in an intact vertebrate organism, we have adapted the MS2 RNA-labeling system to zebrafish. By using this binary system to coexpress a fluorescent MS2 bacteriophage...